Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of structured lipid with long carbon chain and low calorie

A low-calorie, long-carbon-chain technology, applied in the field of preparation of long-carbon-chain low-calorie structured lipids, can solve problems such as high prices and increased production costs, and achieve low cost and simple experimental operations

Inactive Publication Date: 2014-09-03
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
View PDF2 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on low-calorie lipid structure at home and abroad focuses on long-short chain fatty acid esters, medium-carbon chain fatty acid esters, and diglycerides. However, because the reaction raw material requires a single fatty acid, its price is relatively high, which increases production costs.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Accurately weigh 0.115g of glycerin and 0.5g of rice bran wax and dissolve them in 6.15mL of isooctane and tert-amyl alcohol (7:3, v / v) solvent, place on a magnetic stirrer at 200rpm / min, and stir at 50 Heat and stir at ℃ until the reaction substrate is evenly mixed; take 0.34g Novozyme435 (Novozyme product), stir and react at 65℃ for 36h;

[0024] (2) The above-mentioned mixed product is filtered, dried, washed with water, and dried again to obtain a preliminary mixed product;

[0025] (3) Take 1 g of the preliminary mixed product obtained and separate each glyceride by column chromatography, wherein n-hexane, diethyl ether (85:15, v / v) 170mL elute triglyceride, n-hexane, diethyl ether (50: 50, v / v) 150mL to elute diglyceride, diethyl ether, methanol (90:10, v / v) 150mL to elute monoglyceride, after the elution is completed, the solvent can be removed by rotary evaporation to obtain dry triglyceride, glycerol Diester, monoglyceride products, the structural triglyce...

Embodiment 2

[0028] (1) Accurately weigh 0.173g of glycerin and 0.5g of rice bran wax and dissolve them in 6.73mL of isooctane and tert-amyl alcohol (7:3, v / v) solvent, place on a magnetic stirrer at 200rpm / min, and stir at 70 Heat and stir at ℃ until the reaction substrate is evenly mixed, take 0.276g Novozyme435 (Novozyme product), stir and react at 60℃ for 22.4h;

[0029] (2) The above-mentioned mixed product is filtered, dried, washed with water, and dried again to obtain a preliminary mixed product;

[0030] (3) Take 1 g of the preliminary mixed product obtained and separate each glyceride by column chromatography, wherein n-hexane, diethyl ether (85:15, v / v) 170mL elute triglyceride, n-hexane, diethyl ether (50: 50, v / v) 150mL to elute diglyceride, diethyl ether, methanol (90:10, v / v) 150mL to elute monoglyceride, after the elution is completed, the solvent can be removed by rotary evaporation to obtain dry triglyceride, glycerol Diacylglyceride and monoglyceride products, the struc...

Embodiment 3

[0033] (1) Accurately weigh 0.385g of glycerin and 0.5g of rice bran wax and dissolve them in 8.85mL of isooctane and tert-amyl alcohol (7:3, v / v) solvent, place them on a magnetic stirrer at 200rpm / min, and stir at 60 Heat and stir at ℃ until the reaction substrate is evenly mixed, take 0.726g Novozyme435 (Novozyme product), stir and react at 60℃ for 6h;

[0034] (2) The above-mentioned mixed product is filtered, dried, washed with water, and dried again to obtain a preliminary mixed product;

[0035] (3) Take 1 g of the preliminary mixed product obtained and separate each glyceride by column chromatography, wherein n-hexane, diethyl ether (85:15, v / v) 170mL elute triglyceride, n-hexane, diethyl ether (50: 50, v / v) 150mL to elute diglyceride, diethyl ether, methanol (90:10, v / v) 150mL to elute monoglyceride, after the elution is completed, the solvent can be removed by rotary evaporation to obtain dry triglyceride, glycerol Diester, monoglyceride products, the structural mon...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a preparation method of a structured lipid with a long carbon chain and low calorie. The preparation method is mainly characterized by comprising the steps of dissolving glycerin and rice bran wax (of which the mol ratio is (2:1)-(7:1)) into isooctane and tert-amyl alcohol solvents (of which the volume ratio is 7:3) in a concentration of 10%; placing on a magnetic stirrer with a speed of 200rpm / min; heating at 50-70 DEG C, and stirring until substrates are uniformly mixed; adding 4-10% of lipase in percentage by weight, and reacting for 6-36 hours; after completing reaction, removing lipase in a product by virtue of filtration, removing organic solvents by virtue of rotary evaporation, then removing unreacted glycerin by virtue of water washing, and performing vacuum drying to obtain a powdered solid product. The preparation method disclosed by the invention is low in cost and simple in experimental operation, can be used for respectively obtaining triglyceride, diglyceride and monoglyceride by controlling different reaction conditions, and can be used for determining applications of the product according to the characteristics of melting points.

Description

technical field [0001] The invention relates to the technical field of low-calorie functional oils, in particular to a method for preparing lipids with long carbon chains and low-calorie structures. Background technique [0002] The reason why long-carbon-chain saturated fatty acids can synthesize low-calorie lipids is that their melting point is higher than body temperature, and the glycerides formed are not easy to form chylomicrons and micelles, so they are difficult to be digested and absorbed by the human body. According to reports, there are currently more than 1 billion overweight adults in the world, of which at least 300 million are clinically obese, and many chronic diseases induced by it are also increasing. There are several reasons for obesity, such as genes, over-intake and under-consumption, among which high-oil, high-calorie and high-fat diet is one of the main causes of obesity. At present, domestic and foreign research on low-calorie lipid structure focuse...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12P7/64
Inventor 李昌模邓盈盈王硕
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com