Preparation method of structured lipid with long carbon chain and low calorie

A low-calorie, long-carbon-chain technology, applied in the field of preparation of long-carbon-chain low-calorie structured lipids, can solve problems such as high prices and increased production costs, and achieve low cost and simple experimental operations

Inactive Publication Date: 2014-09-03
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on low-calorie lipid structure at home and abroad focuses on long-short chain fatty acid esters, medium-carbon chain fatty acid este

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0022] Example 1

[0023] (1) Accurately weigh 0.115g of glycerin and 0.5g of rice bran wax, dissolve them in 6.15mL isooctane, tert-amyl alcohol (7:3, v / v) solvent, and place them on a magnetic stirrer at 200 rpm / min. Heat and stir at ℃ until the reaction substrates are evenly mixed; Take 0.34g Novozyme435 (product of Novozymes), stir and react at 65℃ for 36h;

[0024] (2) Filtering, drying, washing, and then drying the above-mentioned mixed product to obtain a preliminary mixed product;

[0025] (3) Weigh 1g of the prepared preliminary mixed product and separate each glyceride by column chromatography. Among them, 170 mL of n-hexane, ether (85:15, v / v) elutes triglycerides, n-hexane, ether (50: 50, v / v) 150mL to elute diglycerides, and 150mL of ether and methanol (90:10, v / v) to elute monoglycerides. After elution, the solvent can be removed by rotary evaporation to obtain dry triglycerides and glycerol. Di-ester and monoglyceride products, the structure of triglycerides obtained...

Example Embodiment

[0027] Example 2

[0028] (1) Accurately weigh 0.173g of glycerin and 0.5g of rice bran wax, dissolve them in 6.73mL of isooctane and tert-amyl alcohol (7:3, v / v), and place them on a magnetic stirrer at 200rpm / min. Heat and stir at ℃ until the reaction substrates are evenly mixed, take 0.276g Novozyme435 (Novozymes product), and stir at 60℃ for 22.4h;

[0029] (2) Filtering, drying, washing, and then drying the above-mentioned mixed product to obtain a preliminary mixed product;

[0030] (3) Weigh 1g of the prepared preliminary mixed product and separate each glyceride by column chromatography. Among them, 170 mL of n-hexane, ether (85:15, v / v) elutes triglycerides, n-hexane, ether (50: 50, v / v) 150mL to elute diglycerides, and 150mL of ether and methanol (90:10, v / v) to elute monoglycerides. After elution, the solvent can be removed by rotary evaporation to obtain dry triglycerides and glycerol. Diglyceride and monoglyceride products, the structure of the diglyceride obtained in ...

Example Embodiment

[0032] Example 3

[0033] (1) Accurately weigh 0.385g of glycerin and 0.5g of rice bran wax, dissolve them in 8.85mL of isooctane, tert-amyl alcohol (7:3, v / v) solvent, and place them on a magnetic stirrer at 200rpm / min. Heat and stir at ℃ until the reaction substrate is evenly mixed, take 0.726g of Novozyme435 (Novozymes product), and stir for 6h at 60℃;

[0034] (2) Filtering, drying, washing, and then drying the above-mentioned mixed product to obtain a preliminary mixed product;

[0035] (3) Weigh 1g of the prepared preliminary mixed product and separate each glyceride by column chromatography. Among them, 170 mL of n-hexane, ether (85:15, v / v) elutes triglycerides, n-hexane, ether (50: 50, v / v) 150mL to elute diglycerides, and 150mL of ether and methanol (90:10, v / v) to elute monoglycerides. After elution, the solvent can be removed by rotary evaporation to obtain dry triglycerides and glycerol. Di-ester and monoglyceride products, the structure of monoglyceride obtained in th...

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PUM

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Abstract

The invention relates to a preparation method of a structured lipid with a long carbon chain and low calorie. The preparation method is mainly characterized by comprising the steps of dissolving glycerin and rice bran wax (of which the mol ratio is (2:1)-(7:1)) into isooctane and tert-amyl alcohol solvents (of which the volume ratio is 7:3) in a concentration of 10%; placing on a magnetic stirrer with a speed of 200rpm/min; heating at 50-70 DEG C, and stirring until substrates are uniformly mixed; adding 4-10% of lipase in percentage by weight, and reacting for 6-36 hours; after completing reaction, removing lipase in a product by virtue of filtration, removing organic solvents by virtue of rotary evaporation, then removing unreacted glycerin by virtue of water washing, and performing vacuum drying to obtain a powdered solid product. The preparation method disclosed by the invention is low in cost and simple in experimental operation, can be used for respectively obtaining triglyceride, diglyceride and monoglyceride by controlling different reaction conditions, and can be used for determining applications of the product according to the characteristics of melting points.

Description

technical field [0001] The invention relates to the technical field of low-calorie functional oils, in particular to a method for preparing lipids with long carbon chains and low-calorie structures. Background technique [0002] The reason why long-carbon-chain saturated fatty acids can synthesize low-calorie lipids is that their melting point is higher than body temperature, and the glycerides formed are not easy to form chylomicrons and micelles, so they are difficult to be digested and absorbed by the human body. According to reports, there are currently more than 1 billion overweight adults in the world, of which at least 300 million are clinically obese, and many chronic diseases induced by it are also increasing. There are several reasons for obesity, such as genes, over-intake and under-consumption, among which high-oil, high-calorie and high-fat diet is one of the main causes of obesity. At present, domestic and foreign research on low-calorie lipid structure focuse...

Claims

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Application Information

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IPC IPC(8): C12P7/64
Inventor 李昌模邓盈盈王硕
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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