Shigella flexner strain capable of efficiently degrading fomesafen
A technology of Shigella flexneri and fomesafen, which is applied in the field of Shigella flexneri strains, can solve the problems of few data reports and achieve efficient degradation effect
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specific Embodiment approach 1
[0014] Embodiment 1: The Shigella flexneri strain that efficiently degrades fomesafen in this embodiment is Shigella flexneri FB5 (Shigella flexneri FB5), which is preserved in the General Microorganism Center of China Committee for Microbial Culture Collection (CGMCC ), the deposit address is No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing, the date of deposit is December 06, 2010, and the deposit number is CGMCC No.4410.
[0015] In this embodiment, Shigella flexneri FB5 (Shigella flexneri FB5) is a Gram-negative bacterium, rod-shaped, with a cell size of (0.5 μm-0.7 μm)×(0.9 μm-1.6 μm), no spores, no flagella, no Bacterial film; cultured on inorganic salt solid medium, the colony is round and milky white in color.
[0016] Physiological and biochemical identification of Shigella flexneri FB5 (Shigella flexneri FB5) was carried out with reference to the eighth edition of the "Bergery Bacteria Identification Manual" and "Common Bacterial System Identific...
specific Embodiment approach 2
[0019] Embodiment 2: In this embodiment, Shigella flexneri FB5 (Shigella flexneri FB5) is screened from soil polluted by herbicides in Nehe City, Heilongjiang Province, China. Screening was carried out according to the following steps: Take 5g of herbicide-contaminated soil in Nehe City, Heilongjiang Province in a conical flask, add 50mL of normal saline, shake for 30s, filter with 8 layers of gauze, take 1mL of the filtrate and add it to the flask containing 100mg / L fomesulfame In the inorganic salt medium of ether, cultivate at 30°C and 180r / min, increase the concentration of fomesafen by 100mg / L every 7 days, until the concentration of fomesafen is 500mg / L, to obtain an enriched culture solution; Take 100 μL of the enriched culture solution and evenly spread it on the solid inorganic salt medium with a concentration of fomesafen of 500 mg / L for cultivation. After culturing at 30°C for 24 hours, pick the well-growing colony and purify it to obtain Shiga flexneri Bacillus FB5...
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