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Shigella flexner strain capable of efficiently degrading fomesafen

A technology of Shigella flexneri and fomesafen, which is applied in the field of Shigella flexneri strains, can solve the problems of few data reports and achieve efficient degradation effect

Active Publication Date: 2011-09-07
海林市中农国泰生物科技有限公司
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  • Claims
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Problems solved by technology

However, there are few reports on the microbial degradation of fomesafen

Method used

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  • Shigella flexner strain capable of efficiently degrading fomesafen
  • Shigella flexner strain capable of efficiently degrading fomesafen
  • Shigella flexner strain capable of efficiently degrading fomesafen

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specific Embodiment approach 1

[0014] Embodiment 1: The Shigella flexneri strain that efficiently degrades fomesafen in this embodiment is Shigella flexneri FB5 (Shigella flexneri FB5), which is preserved in the General Microorganism Center of China Committee for Microbial Culture Collection (CGMCC ), the deposit address is No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing, the date of deposit is December 06, 2010, and the deposit number is CGMCC No.4410.

[0015] In this embodiment, Shigella flexneri FB5 (Shigella flexneri FB5) is a Gram-negative bacterium, rod-shaped, with a cell size of (0.5 μm-0.7 μm)×(0.9 μm-1.6 μm), no spores, no flagella, no Bacterial film; cultured on inorganic salt solid medium, the colony is round and milky white in color.

[0016] Physiological and biochemical identification of Shigella flexneri FB5 (Shigella flexneri FB5) was carried out with reference to the eighth edition of the "Bergery Bacteria Identification Manual" and "Common Bacterial System Identific...

specific Embodiment approach 2

[0019] Embodiment 2: In this embodiment, Shigella flexneri FB5 (Shigella flexneri FB5) is screened from soil polluted by herbicides in Nehe City, Heilongjiang Province, China. Screening was carried out according to the following steps: Take 5g of herbicide-contaminated soil in Nehe City, Heilongjiang Province in a conical flask, add 50mL of normal saline, shake for 30s, filter with 8 layers of gauze, take 1mL of the filtrate and add it to the flask containing 100mg / L fomesulfame In the inorganic salt medium of ether, cultivate at 30°C and 180r / min, increase the concentration of fomesafen by 100mg / L every 7 days, until the concentration of fomesafen is 500mg / L, to obtain an enriched culture solution; Take 100 μL of the enriched culture solution and evenly spread it on the solid inorganic salt medium with a concentration of fomesafen of 500 mg / L for cultivation. After culturing at 30°C for 24 hours, pick the well-growing colony and purify it to obtain Shiga flexneri Bacillus FB5...

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Abstract

The invention provides a Shigella flexner strain capable of efficiently degrading fomesafen and relates to a Shigella flexner strain. The Shigella flexner strain provided by the invention is capable of efficiently degrading the fomesafen, thereby providing a theoretical basis and a microbial resource basis for the environmental restoration and degradation mechanism researches of the diphenyl ether herbicide. The Shigella flexner strain is Shigella flexner FB5, wherein the strain was preservd in the China General Microbiological Culture Collection Center with the collection number of CCTCC NO: 4410 on December 06, 2010. The Shigella flexner FB5 can be used for efficiently degrading the fomesafen and also restoring the soil containing residual fomesafen.

Description

technical field [0001] The present invention relates to a Shigella flexneri bacterial strain. Background technique [0002] Fomesafen (common name: fomesafen, trade name: Huwei), is a diphenyl ether contact herbicide developed by the British Zeneca Company, which is used for the control of broad-leaved weeds in crops such as soybeans, fruit trees, and rubber plantations. It is widely used because of its good weed control effect. Fomesafen is used in a large amount and area across the country. Its use before and after soybean seedlings can quickly absorb the weeds into the leaves, destroy their photosynthesis, and cause the weeds to wither and die quickly. However, this herbicide will continue to act for a long time in the soil, and the medicine remaining in the soil will cause damage to the crop rotation due to its large phytotoxicity. [0003] Microbial degradation is the main decomposition method of diphenyl ether herbicides in soil. At present, the degradation of fomes...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02B09C1/10C12R1/01A62D101/04A62D101/28
Inventor 杨峰山刘春光刘亮刘丽萍肖延臣刘爽
Owner 海林市中农国泰生物科技有限公司
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