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Method for assisting in identifying cynomorium plant and special primers thereof

An auxiliary identification and plant technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems that have not yet been seen in Cynomorium molecular biological identification methods, and achieve low dosage, high efficiency, and stability high effect

Inactive Publication Date: 2011-09-14
INNER MONGOLIA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the molecular biological identification methods of important medicinal materials such as ginseng, astragalus, and Cistanche tubulosa have been reported, but there is no report on the molecular biological identification methods of Cynomorium.

Method used

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  • Method for assisting in identifying cynomorium plant and special primers thereof
  • Method for assisting in identifying cynomorium plant and special primers thereof
  • Method for assisting in identifying cynomorium plant and special primers thereof

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Embodiment 1

[0027] Embodiment 1, identification of Chinese herbal medicine Rhizoma Cynomorium

[0028] Method I

[0029] 1. Genomic DNA extraction from different samples

[0030] Cynomorium songaricum Rupr. collected from Urumqi, Xinjiang (purchased from Xinjiang Huashi Wankang Chain Pharmacy Co., Ltd. Hetian 2nd Street Store), Cynomorium songaricum Rupr. collected from Anxi County, Gansu (purchased from Gansu Suoyangchun Health Drink Co., Ltd.), Cynomorium songaricum Rupr. collected from Haixi, Qinghai (purchased from the medicinal material wholesale market in Delingha, Haixi Prefecture, Qinghai), Cynomorium songaricum Rupr collected from Hangjin Banner, Inner Mongolia .) (The public can obtain from Inner Mongolia University, the non-patent literature that has recorded this material is: Ma LJ, Chen GL, Jin SW, Wang CX, 2008.The anti-aging effect and the chemical constituents of Cynomorium songaricum Rupr.ACTAHORTICULTURAE.765 : 23-30. Inner Mongolia University) and Cynomorium songaricu...

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Abstract

The invention discloses a method for assisting in identifying a cynomorium plant and special primers thereof. A primer pair for identifying the cynomorium plant comprises the primer as shown in sequence 1 in a sequence table and the primer as shown in sequence 2 in the sequence table. The method for assisting in identifying the cynomorium plant comprises the following steps: taking genome DNA (deoxyribonucleic acid) of the plant to be identified as a template, and using the primer pair described in claim 1 to perform PCR (polymerase chain reaction) amplification so as to get a PCR amplification product; and sequencing and detecting the PCR amplification product, and determining that the plant to be identified is a candidate of the cynomorium plant if the PCR amplification product is a 1064bp fragment. By adopting the method, not only subjective factors in morphological identification of traditional Chinese medicinal materials can be compensated, but also the characteristics of small using quantity, high efficiency, high stability, accuracy and reliability are simultaneously realized, thereby having important significance for standardizing the medicinal materials of cynomorium.

Description

technical field [0001] The invention relates to a method for assisting identification of Cynomorium genus plants in the field of biotechnology and special primers thereof. Background technique [0002] Cynomorium is the fleshy stem of the plant Cynomorium songaricum Rupr. of Cynomoriaceae (Cynomoriaceae). The plant Cynomorium is also known as Ulan high waist, elixir, and gold. It is a perennial parasitic herb , parasitic exclusively on the roots of Nitraria L. plants of the family Zygophyllaceae, Cynomorium is mainly produced in the semi-desert or desert areas of Inner Mongolia, Xinjiang, Gansu, Qinghai, Ningxia and other places. It is an important traditional Chinese medicine and Herbal plants. [0003] Cynomorium yang is warm and sweet. It is recorded in "Compendium of Materia Medica": Cynomorium yang can nourish yin and qi, benefit essence and blood, relieve stool, moisten dryness and nourish tendons, and treat flaccidity and weakness. Modern medical research shows that...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 陈贵林刘广达王丽红冯丹陈青
Owner INNER MONGOLIA UNIVERSITY