Inspection method capable of inspecting traditional Chinese medicine injection tannins
An inspection method and injection technology, applied in the direction of testing pharmaceutical preparations, material inspection products, etc., can solve problems such as differences in molecular weight and physical and chemical properties, and achieve the effect of high sensitivity and specificity, and strong anti-interference ability
Inactive Publication Date: 2011-10-19
YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
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Problems solved by technology
[0005] The tannins in traditional Chinese medicine injections are mixtures, and the molecular weight of tannins is an uncertain value. Therefore, the molecular weight and physical and chemical properties may be quite different, and it is difficult to check by instrumental analysis methods such as HPLC and / or mass spectrometry.
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[0029] Implementation example 1
[0030] Using the protein-coated PVDF membrane to establish a method for the inspection of tannins in traditional Chinese medicine injections by spotting, and to determine the inspection limit
[0031] Take a piece of PVDF membrane, wet it with methanol, soak it in 10% egg white solution, shake it at room temperature for 3 hours, take it out and air dry it for later use.
[0032] Prepare 5ml of pyrogallic acid solution with a concentration of 20mg / ml in distilled water, leave it at room temperature naturally, and wait for the absorbance (410nm, d=1cm) of the solution to reach 3 or more as a tannin standard stock solution. Take 8 1.5ml test tubes numbered 1-8. Add 1.5ml of standard tannin stock solution to tube 1. Then add 1ml of distilled water to each of No. 2-8 tubes, add 0.5ml of the solution from No. 1 tube to No. 2 tube, mix well, then take 0.5ml and add it to No. 3 tube, and then prepare to No. 7 tube. That is, a group of tannin standar...
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The invention relates to an inspection method capable of inspecting traditional Chinese medicine injection tannins, and is used for inspecting the tannin impurities of a traditional Chinese medicine injection to control the quality of the traditional Chinese medicine injection. The inspection method provided by the invention is characterized by comprising the following steps: 1) coating a protein absorption membrane with proteins; 2) enabling a traditional Chinese medicine injection solution to be in contact with the protein coated membrane; 3) washing protein coated membrane by utilizing the solution containing organic solvents; and 4) observing the color of sample application speckles and judging the tannins in the traditional Chinese medicine injection. In the inspection method provided by the invention, the concentration of the tannins in the traditional Chinese medicine injection which are inspected by adopting a sample application mode and can reach 0.75-0.25mg / ml, can reach 0.07mg / ml by adopting a filtering mode; the inspection limit quantity is lower than the quantity in the Pharmacopeia (first chapter) of the People's Republic of China (PRC) in 2010 edition; and simultaneously, the interference resistance is strong and is suitable for the inspection of the traditional Chinese medicine injection tannins.
Description
Technical field [0001] The invention relates to the field of impurity inspection of traditional Chinese medicine injections, which is used for tannin impurities in traditional Chinese medicine injections to control the quality of traditional Chinese medicine injections. technical background [0002] Traditional Chinese medicine injections were first produced in the 1940s by scholars such as Qian Xinzhong. In China, traditional Chinese medicine injections have been widely used clinically and have been recognized by the industry. The 2010 edition of "The Pharmacopoeia of the People's Republic of China" (referred to as "Chinese Pharmacopoeia") includes single and compound injections such as Dengzhan Xixin, Qingkailing, and Zhichuanling. Traditional Chinese medicine injections have played an important role in promoting the development of traditional Chinese medicine formulations, expanding the scope of application of traditional Chinese medicine, and serving the health of the g...
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Inventor 段为钢柯瑾李奇峰林青殷华
Owner YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE