Reagent for determining total bilirubin by sodium nitrite oxidation method

A technology of sodium nitrite and total bilirubin, which is applied in the direction of color/spectral characteristic measurement and analysis through chemical reaction of materials to achieve the effects of stable performance, scientific components and easy production

Active Publication Date: 2011-10-26
郑州蓝跃生物科技股份有限公司
View PDF4 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In view of the above situation, in order to overcome the defects of the prior art, the purpose of the present invention is to provide a reagent for the determination of total bilirubin by sodium nitrite oxidation, which can effectively solve the problem of wide linear range, strong anti-interference, standardized response curve, and can quickly reach the end point. And the accuracy and precision of the determination are high, and it can be practically applied without the problem of polluting the environment

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Reagent for determining total bilirubin by sodium nitrite oxidation method
  • Reagent for determining total bilirubin by sodium nitrite oxidation method
  • Reagent for determining total bilirubin by sodium nitrite oxidation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] In the specific implementation of the present invention, the reagent 1 is composed of 4.41 g of trisodium citrate, 17.85 g of citric acid, 0.25 ml of polyethylene glycol octyl phenyl ether, and 0.37 g of disodium ethylenediamine tetraacetate. Distilled water to 1000ml, mixed evenly to form, the pH value is 2.9; said reagent 2 is composed of 0.32g sodium nitrite, 0.1g sodium hydroxide, 0.2g sodium carbonate, added distilled water to 1000ml, mixed uniformly, the pH value is 12.

[0013] The reagent for measuring total bilirubin by the sodium nitrite oxidation method of this embodiment is based on the following principle to measure total bilirubin: sodium nitrite can oxidize direct bilirubin and indirect bilirubin. Bilirubin is directly proportional, and the decrease of absorbance at 450nm is measured to calculate the content of total bilirubin in serum.

[0014] Temperature 37°C; cuvette optical path 1.0cm wavelength 450nm

[0015] 10 μl each of the sample and the stand...

Embodiment 2

[0022] In the specific implementation of the present invention, it is also possible that the reagent 1 consists of 2.25 g of trisodium citrate, 19.80 g of citric acid, 0.10 ml of polyethylene glycol octylphenyl ether, 0.20 g of disodium edetate, and distilled water to 1000ml, the pH value is 2.2; Reagent 2 consists of 0.14g sodium nitrite, 0.05g sodium hydroxide, 0.10g sodium carbonate, and distilled water to 1000ml, the pH value is 11.

[0023] The reagent of this embodiment 2 measures the method for total bilirubin with embodiment 1, repeats no more.

Embodiment 3

[0025] In the specific implementation of the present invention, it is also possible that the reagent 1 is composed of 5.26 g of trisodium citrate, 16.40 g of citric acid, 1.0 ml of polyethylene glycol octyl phenyl ether, and 0.65 g of disodium edetate. , add distilled water to 1000ml, pH value is 3.6; Reagent 2 consists of 0.56g sodium nitrite, 0.15g sodium hydroxide, 0.30g sodium carbonate, add distilled water to 1000ml, pH value is 13.

[0026] The reagent of this embodiment 3 measures the method for total bilirubin with embodiment 1, repeats no more.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a reagent for determining total bilirubin by a sodium nitrite oxidation method, which can effectively solve present problems and has a wide linear range, strong interference immunity, a standard reaction curve, a rapidly-reached end point, high accuracy and precision of determination, and the capability of practical application without environmental pollution. The preparation method of the reagent is that: a reagent 1 is prepared by adding water into 2.25-5.26 g of trisodium citrate, 16.40-19.80 g of citric acid, 0.10-1.0 ml of polyethyleneglycol octyl phenyl ether, 0.20-0.65 g of disodium ethylene diamine tetraacetate to obtain 1000 ml, and mixing the mixture, wherein the reagent 1 has a pH of 2.2-3.6; a reagent 2 is prepared by adding water into 0.14-0.56 g of sodium nitrite, 0.05-0.15 g of sodium hydroxide, 0.10-0.30 g of sodium carbonate to obtain 1000 ml, and mixing the mixture, wherein the reagent 2 has a pH of 11-13. The invention has scientific components, is easy to produce, has low cost, stable performance, and long shelf life, is accurate in determination, and has no environmental pollution.

Description

1. Technical field [0001] The invention relates to a reagent for measuring total bilirubin in serum, plasma or urine, in particular to a reagent for determining total bilirubin by a sodium nitrite oxidation method. 2. Background technology [0002] Total bilirubin (TBil) is the sum of conjugated (direct) and unconjugated (indirect) bilirubin in the human body. Increased total bilirubin, usually due to biliary obstruction, hepatitis, cirrhosis, hemolytic syndrome, and certain inherited enzyme deficiencies. In recent years, some studies have shown that lower than normal blood bilirubin is often related to coronary heart disease. Therefore, the determination of total bilirubin in human serum, plasma or urine is one of the most routine items in medical testing. [0003] There are currently three main reagents for determining total bilirubin: reagents for the determination of total bilirubin by the diazo method, reagents for the determination of total bilirubin by the enzymatic ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/75G01N21/31
Inventor 牛好伟王玉金王玉珠边倩茹李瑾
Owner 郑州蓝跃生物科技股份有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap