Fermentation culture medium and fermentation method for producing pentostatin by fermentation of streptomyces antibioticus

Abstract

The invention provides a fermentation culture medium and a fermentation method for producing pentostatin by fermentation of streptomyces antibioticus. The fermentation culture medium comprises a carbon source and a nitrogen source, wherein based on the total weight of the culture medium, the content of the carbon source is 0.5-14%, and the content of the nitrogen source is 0.5-10%; and the carbon source is one or more of glucose, dextrin and wheat starch, and the nitrogen source is one or more of fine bean pulp powder, soy protein, soybean cake powder, yeast soaking powder and cotton seed cake powder. According to the invention, the yield of pentostatin is greatly improved through optimizing the fermentation parameters of the fermentation culture medium, such as components and proportioning of the carbon and nitrogen sources, culture temperature, pH and the like. By using the fermentation production method of pentostatin, the fermentation unit of pentostatin is greatly improved, and the fermentation method is suitable for large scale production of pentostatin.

Description

technical field

[0001] The invention belongs to the technical field of industrial microorganisms, and in particular relates to a novel fermentation medium for producing pentostatin by fermentation of antibiotic streptomyces and a corresponding fermentation method. Background technique

[0002] In 1974, Woo et al. from Warner Lambert Company discovered an adenosine deaminase inhibitor-pentostatin from the fermentation broth of Streptomyces antibioticus. In 1992, pentostatin was approved by the FDA for the treatment of stem cell leukemia (hairy cell leukemia, HCL). With the increase of its clinical application, the market demand increases. However, there are very few reports on the production of pentostatin. The only reported fermentation process is found in US3923785. In this patent, Streptomyces NRRL3238 is used as the production strain, and a relatively simple culture medium and culture method are mainly reported. , the purpose of which is to isolate pentostatin from the ...