Living body imaging method for monitoring temperature change in plant flower bud differentiation or flowering process

Abstract

The invention discloses a living body imaging method for monitoring a temperature change in a plant flower bud differentiation or flowering process, which comprises the following steps of: 1, acquiring a flower bud or flower organ image of a living body plant to be monitored by adopting an infrared non-contact thermal imaging system; and 2, obtaining temperatures of different sites of the flower bud or flower organ of the living body plant to be monitored according to the acquired image obtained in the step 1 so as to monitor the induced thermogenesis of the plant flower bud differentiation or flowering. Experiments prove that the method has the advantages of simpleness and rapidness in operation so that forward test analysis steps of sampling, separating and the like in plant observationare omitted, and good imaging effect so that living body observation can be carried out on thermogenesis of flower organs of the plant under the condition that outside environment interference such as rain, snow and the like is avoided and the temperature difference discrimination is 0.1DEG C.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an in vivo imaging method for monitoring temperature changes during plant flower bud differentiation or flowering. Background technique [0002] The mechanism of reproductive development is an important feature that distinguishes a species from other species, and it is also a concrete manifestation of the co-evolution of the original population adapting to the environment during the historical evolution process. As the sexual reproductive organs of flowering plants, the floral organs play a vital role in the process of plant reproduction and development. Therefore, in the process of reproductive development of flowering plants, from the differentiation of flower buds to the maturity of flower organs, The entire developmental process from germination and growth of mature pollen on stigma to double fertilization to seed maturity has become the key field of plant reproductive developmen...

AI Technical Summary

Problems solved by technology

Due to the lack of effective experimental instruments and technical means for the instantaneous capture of the thermogenic effect of flowering and the measurement of the temperature inside the plant during plant reproduction and development, most of the existing research is to obtain experimental data from chemically fixed "dead cells". This may be very different from the results obtained in in vivo measurements, and because the subtle heat generated by respiratory metabolism in plant reproduction and development cannot be captured in a timely and effective manner. The technology has not been reported, which has led to the relatively lagging behind in vivo research on reproductive and development mechanisms such as flowering mechanisms. So far, researchers have not yet made substantial progress in revealing the mechanism of flowering and heat generation in reproductive and development mechanisms.

[0004] Infrared technology and infrared imaging have been used in construction, factory inspection and other fields since they were used, but their application in the field of plant research has not been reported, especially in the experimental field of plant reproductive development and floral organ differentiation. Not yet reported

Images