Method for preparing meat flavor precursor by controlling enzymolysis-mild heating oxidation of fats
A technology of heating oxidation and meat flavor, which is applied in the field of meat flavor precursors, can solve the problems of hala taste, high energy consumption, excessive oxidation, etc., and achieve mellow and full flavor, distinctive aroma and full flavor Effect
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Embodiment 1
[0019] 70g of refined chicken fat was added to 30g of 0.1mol / L pH=6 phosphate buffer (substrate concentration 70%), the mixture was placed in an enzymolysis reactor, stirred slowly, and 1200U / g was added after the chicken fat was melted The substrate lipase MER was hydrolyzed at 37°C for 2 hours, and the enzyme was inactivated to obtain the hydrolyzed product.
[0020] Add the above chicken fat enzymatic hydrolyzate into a 250mL four-necked flask equipped with an electric stirrer, a mercury thermometer, an air inlet tube and a reflux condenser, and stir the reaction with air at 60°C, the stirring speed is 100r / min, and the air flow rate is 60L / h, stop the reaction after 3h of oxidation, cool down and let stand to stratify. The oxidized chicken fat in the upper layer is the product. Its peroxide value is 81.39meq / kg fat, and its acid value is 39.68mgKOH / g fat.
Embodiment 2
[0022] Add 33g of 0.05mol / L pH=6.5 phosphate buffer solution (substrate concentration 60%) to 50g of refined lard, place the mixture in an enzymolysis reactor, stir slowly until the lard is melted, add 1000U / g bottom The lipase DF was hydrolyzed at 38°C for 3 hours to inactivate the enzyme to obtain the hydrolyzed product.
[0023] Add the above pig fat enzymatic hydrolyzate into a 250mL four-necked flask equipped with an electric stirrer, a mercury thermometer, an air inlet tube and a reflux condenser, and stir the reaction with air at 80°C, the stirring speed is 150r / min, and the air flow rate is 75L / h, stop the reaction after 3h of oxidation, cool and stand for stratification, the upper layer of oxidized lard is the product, its peroxidation value is 120.36meq / kg fat, and its acid value is 46.29mgKOH / g fat.
Embodiment 3
[0025] Add 20 g of 0.05 mol / L pH=6 phosphate buffer (substrate concentration 80%) to 80 g of refined tallow, place the mixture in an enzymolysis reactor, stir slowly until the tallow is melted, then add 2500 U / g of substrate lipase MER was hydrolyzed at 40°C for 5 hours, and the enzyme was inactivated to obtain the hydrolyzed product.
[0026] Add the above tallow enzymatic hydrolyzate into a 250mL four-neck flask equipped with an electric stirrer, a mercury thermometer, an air inlet tube and a reflux condenser, and stir the reaction with air at 100°C. The stirring speed is 200r / min, and the air flow rate is 80L / min. h, stop the reaction after 2 hours of oxidation, cool and stand for stratification, the oxidized tallow in the upper layer is the product, its peroxidation value is 111.96meq / kg fat, and its acid value is 51.24mgKOH / g fat.
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