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Immune chromatography test paper for detecting clenbuterol hydrochloride and preparation method thereof

A technology of clenbuterol hydrochloride and immunochromatographic test paper, which can be used in measurement devices, analytical materials, instruments, etc., and can solve the problems of long color development time, cumbersome and complicated, and unable to provide enough magnetic resonance signals.

Inactive Publication Date: 2011-11-23
SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these preparation and modification methods are often cumbersome and complicated, and the obtained superparamagnetic composite particles cannot meet the requirements of LFIAs in terms of size, biocompatibility, saturation magnetic strength, external magnetic field response speed, stability, and labeling efficiency. The size is mostly between 200 and 300nm. Due to the large size of the magnetic beads, the swimming time on the test paper is slow and the color development time is long; and the particles that are too small cannot provide sufficient magnetic resonance signals; in addition, there are biological Unstable compatibility and easy aggregation of magnetic beads; these shortcomings limit the application of superparamagnetic composite particles in LFIAs

Method used

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  • Immune chromatography test paper for detecting clenbuterol hydrochloride and preparation method thereof
  • Immune chromatography test paper for detecting clenbuterol hydrochloride and preparation method thereof
  • Immune chromatography test paper for detecting clenbuterol hydrochloride and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0084] Example 1. Preparation of magnetically labeled rapid detection test paper for clenbuterol hydrochloride residues

[0085] (1) Preparation of clenbuterol hydrochloride antibody labeled with superparamagnetic composite particles

[0086] The particle size is 100nm, the magnetic saturation intensity is 40emu / g, the corresponding external magnetic field response speed is 20 seconds, and the superparamagnetic composite particle (superparamagnetic Fe 3 o 4 Nanoparticles) (purchased from Shenzhen Thales Technology Co., Ltd., catalog number MP-2) labeled clenbuterol hydrochloride antibody.

[0087] The specific method is:

[0088] 1) Take 2.5mg of the above-mentioned magnetic particles, wash them with 0.1M MES buffer (weigh 1.066g MES, 0.45g NaCl and dissolve them in 50ml pure water, adjust the pH to 4.7), separate and enrich them with a 0.4T magnetic rack, and use 1ml of MES buffer with a concentration of 0.1M and a pH value of 4.7 was resuspended, and 0.96mg (final concent...

Embodiment 2

[0101] Example 2, Detection of Immunochromatography Test Paper Sensitivity, Cross Reaction and Accuracy of Clenbuterol Hydrochloride Residues

[0102] 1. Determination of sensitivity

[0103] Weigh an appropriate amount of clenbuterol hydrochloride (Beijing Hengyuan Qitian Chemical Technology Research Institute, catalog number: 30229CDCT-C11668550), and prepare a 100ug / ml standard stock solution with 0.01M HCl. Dilute and prepare standard solutions of 0, 0.001, 0.005, 0.01, 0.05, 0.1, 1, 5, 10 ug / L with 0.02M PBS (pH=7.4), add the detection clenbuterol hydrochloride obtained by Example 1 respectively Immunochromatographic test paper, and read using superparamagnetic resonance detector MAR (Magna BioSciences, 8094-101-01 & 8094-101-02). Detection steps: Return the sample to be tested to room temperature (25°C) before detection, use a precise pipette to take 100 μl of the sample to be tested and drop it vertically and slowly into the sample loading end of the magnetic test stri...

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Abstract

The invention discloses immune chromatography test paper for detecting clenbuterol hydrochloride and a preparation method thereof. The immune chromatography test paper for detecting the clenbuterol hydrochloride in the invention comprises a sample pad containing superparamagnetic composite particle labeled clenbuterol hydrochloride antibody, cellulose nitrate membrane connected at one end of the sample pad, and a water absorbent pad connected at the other end of the cellulose nitrate membrane; the cellulose nitrate membrane is coated with separated detection line and quality control line, the detection line contains a clenbuterol hydrochloride antigen and the quality control line contains an antiantibody capable of being specifically bonded to the clenbuterol hydrochloride antibody. Through the experiment demonstration, the test paper provided the invention has the advantages of high sensitivity and specificity, rapidness, simplicity and convenience in detecting the clenbuterol hydrochloride, and can realize objective detection.

Description

technical field [0001] The invention relates to a detection reagent for clenbuterol hydrochloride residues, in particular to an immunochromatographic test paper for detecting clenbuterol hydrochloride and a preparation method thereof. Background technique [0002] Clenbuterol hydrochloride (CL) is a β-stimulant, which has been illegally added to feed in recent years to increase the lean meat rate of fat animals and accelerate animal growth, because the additive dose is 5-10 times the therapeutic dose. times, so that the residues in animals are high and bring harm to consumers. At present, clenbuterol hydrochloride colloidal gold test paper is mostly used as the detection reagent for on-site screening, but it cannot meet the requirements well due to its low sensitivity. Therefore, it is necessary to develop a more accurate and rapid detection method in the field. [0003] Lateral flow immunochromatographic detection technology (LFIAs) based on antigen-antibody immunological ...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/531
Inventor 马岚卢体康秦智锋袁航吴峰
Owner SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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