Method for establishing peanut regeneration system with young leaf as explant

A technology of explants and peanuts, applied in the biological field, can solve problems such as low regeneration efficiency and achieve good growth effect

Inactive Publication Date: 2011-11-30
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
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  • Claims
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Problems solved by technology

The tissue culture of peanuts began in the 1940s, and in my...

Method used

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  • Method for establishing peanut regeneration system with young leaf as explant
  • Method for establishing peanut regeneration system with young leaf as explant
  • Method for establishing peanut regeneration system with young leaf as explant

Examples

Experimental program
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Embodiment 1

[0028] 1. Determination of bud induction medium and elongation medium using young leaves as explants

[0029] 1. Shell the peanuts, place them in 75% ethanol for 1 minute, discard the ethanol, place them in 0.1% mercuric chloride for 4 minutes, wash them with sterilized water for 3 times, place them on sterile paper until they are dried;

[0030] 2. Use a sterile scalpel to separate the two cotyledons of the peanut, place the cotyledon containing the complete embryo on the basic medium, and cultivate under light;

[0031] 3. Select young and young leaves that have been cultivated for different times, cut off the leaf margins, and place them on the bud induction medium (Table 1) containing TDZ, 6-BA and NAA. left and right explants;

[0032] Table 1 Orthogonal experiment design of bud induction medium

[0033]

[0034] a: Time from sowing to leaf clipping

[0035] 4. Select the young leaves that have been cultivated for 5 days, cut off the leaf margins, place them face up...

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Abstract

The invention relates to a method for establishing a peanut regeneration system with a young leaf as an explant, belonging to the technical field of biology. The method comprises the following steps: based on the young leaf as the explant of the peanut regeneration system, inducing and germinating on a bud induction culture medium containing TDZ (thidiazuron) and NAA (naphthyl acetic acid); promoting adventitious bud to extend on an extending culture medium containing 6-BA (6-benzyl aminopurine) and NAA; culturing to take root on the root growing culture medium containing NAA so as to obtain rooted seedling; and transplanting and cultivating according to a conventional method. In the regeneration system established by the method in the invention, bud inductivity reaches up to about 40%, and bud elongation ratio reaches up to 79%; and subculture growth is good in the regeneration system, thus the regeneration system can become a good regeneration system for gene transformation.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for establishing a peanut regeneration system using young leaves as explants. Background technique [0002] Peanut is one of the important oil crops in the world, and my country's peanut production ranks first in the world. Some biotic and abiotic stresses can affect the yield of peanut, especially soil pests, fungi and bacterial diseases. Although traditional cross-breeding can produce some insect-resistant and disease-resistant lines (Garcia et al., 2006), the genetic diversity of peanut cultivars is low (Kochert et al., 1991), the cycle of cross-breeding is long, and the disease-resistant traits developed It is usually linked with other unexpected traits. Therefore, currently, cultivating peanut varieties by means of molecular breeding has obvious advantages in improving peanut quality. [0003] The efficiency of the regeneration system is the key to the success of gene...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 耿丽丽束长龙张杰宋福平黄大昉
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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