Pacific cod sperm preservation freezing agent and Pacific cod sperm preservation method

A Pacific cod and refrigerant technology, which is applied in the field of refrigerants, can solve the problems of inability to solve the sexual maturity of male and female broodstock, the inability to store for a long time, and the short storage time, etc., to solve the problem of asynchronous sexual maturity of male and female broodstock, market prospects and scientific research Great prospects and long-lasting effects

Inactive Publication Date: 2011-11-30
DALIAN OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, room temperature storage has the characteristics of convenience and simplicity, but the storage time is very short, which cannot solve the problem of asynchronous sexual maturity of male and female broodstock
Although the storage time at low temperature is longer than that at room temperature, many physical and chemical factors such as osmotic pressure and pH value will affect the final storage effect during low temperature storage. At the same time, this method cannot be used for long-term storage.

Method used

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Embodiment Construction

[0017] The following are specific embodiments of the present invention.

[0018] A kind of Pacific cod sperm preservation refrigerant is characterized in that: described refrigerant is made up of freezing diluent and antifreeze two parts, and wherein the component content of freezing diluent is:

[0019] NaCl: 42-48mM, with 45mM as the best value; KHCO 3 : 2.5-3.5mM, 3mM is the best value; CaCl 2 : 2.5-3.5mM, 3mM is the best value; Glucose: 35-45mM, 40mM is the best value; Tris: 1.5-2.5mM, 2mM is the best value; Glutathione: 1.5-2.5mM, 2mM is the best value; bovine serum albumin: 1.5-2.5g / L, 2g / L is the best value;

[0020] After the above-mentioned raw materials are fully and uniformly mixed, use 0.1M HCl to adjust the pH value of the mixture to 6.7-6.9, with 6.8 being the best (the osmotic pressure of the frozen diluent after the above treatment is 150- 155mosM / kg).

[0021] The frozen diluent is prepared and stored at 4°C.

[0022] When long-term preservation of ...

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Abstract

The invention discloses a cryogen for Pacific cod sperm preservation, which is characterized in that: the cryogen is mixed with a freeze diluent and an antifreeze in a ratio of 9:1 (volume ratio), wherein the components of the freeze diluent The contents are: NaCl: 42-48mM, KHCO3: 2.5-3.5mM, CaCl2: 2.5-3.5mM, Glucose: 35-45mM, Tris: 1.5-2.5mM, Glutathione: 1.5-2.5mM, Bovine Serum Albumin: 1.5-2.5g / L, the pH value range of the frozen diluent is: 6.7-6.9; the antifreeze agent in the frozen agent is dimethyl sulfoxide (DMSO). As well as a method for cryopreserving Pacific cod sperm by using the cryogen. The freezing agent and the preservation method can preserve Pacific cod sperm for a long time, and can ensure that the sperm viability can still reach more than 80% after thawing.

Description

technical field [0001] The invention relates to a freezing agent for preserving Pacific cod sperm and a method for using the freezing agent to preserve Pacific cod sperm. Background technique [0002] In the process of artificial fish breeding, problems such as asynchronous sexual maturity of male and female broodstock, few male broodstock, and insufficient semen volume are often encountered, which affect the efficiency and output of fish farming. Fish semen preservation technology can solve this problem. The existing fish semen preservation methods mainly include normal temperature and low temperature (0 to 4 degrees Celsius). Among them, normal temperature preservation has the characteristics of convenience and simplicity, but the preservation time is very short, which cannot solve the problem of asynchronous sexual maturation of male and female broodstock. Although the storage time at low temperature is longer than that at room temperature, many physical and chemical fa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
Inventor 王伟姜志强王华李君丰
Owner DALIAN OCEAN UNIV
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