Pit mud functional bacteria and preparation method thereof

A technology for functional bacteria and pit mud, applied in the field of microbial fermentation, can solve the problems such as no reports of functional bacteria in pit mud, and achieve the effects of facilitating storage and transportation, improving production efficiency, and having broad application prospects.

Active Publication Date: 2011-11-30
LUZHOU PINCHUANG TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are no relevant reports on the

Method used

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preparation example Construction

[0023] a. Preparation of caproic acid bacteria liquid: Caproic acid bacteria strains are rejuvenated and expanded to obtain caproic acid bacterial liquid;

[0024] B, the preparation of cellar mud-enriched bacterial liquid: get the bacterial classification in pit mud (the beneficial bacterial classification in the pit mud in the old pit of the age is more abundant, therefore, preferably the cellar mud of more than 10 years) in the pit mud to carry out Cultivate to obtain the cellar mud-enriched bacterial solution;

[0025] c, preparation of pit mud functional bacteria: caproic acid bacterial liquid mixes with pit mud enriched bacterial liquid, inoculates (in the present invention, all can be inoculated by conventional strain inoculation method) in pit mud functional bacteria culture medium, 32~ Cultivate at 36°C for 22-27 days to obtain the functional bacterial liquid of pit mud;

[0026] Wherein, the ratio of caproic acid bacteria liquid to the strain dry weight in the cella...

Embodiment 1

[0039] Embodiment 1 adopts the method of the present invention to prepare pit mud functional bacteria

[0040] 1. Preparation of caproic acid bacteria solution

[0041] Put caproic acid bacteria into sterile water at a ratio of 1:4. After 8-10 minutes of treatment in a hot water bath at 80°C, inoculate the caproic acid bacteria seeds in the caproic acid bacteria seed culture medium. The culture condition is that the temperature is 34 °C, the pH value is 6, and the culture time is 7-8 days. When the culture solution turns pale yellow and small bubbles rise, and the bacteria are in a vigorous metabolic period, expand the culture.

[0042] Cultivation of caproic acid bacteria liquid for production: Rinse the 10L earthenware pot with water → fermented grain filtrate, sodium acetate and ammonium sulfate (the amount added is half of the container) → add steam and heat to boil, keep for 30 minutes → wait for the altar to cool to 40 ℃, add high-temperature sterilized calcium carbona...

Embodiment 2

[0051] Embodiment 2 adopts the method of the present invention to prepare pit mud functional bacteria

[0052] 1. Preparation of caproic acid bacteria solution

[0053] Put caproic acid bacteria into sterile water at a ratio of 1:4. After 8-10 minutes of treatment in a hot water bath at 80°C, inoculate the caproic acid bacteria seeds in the caproic acid bacteria seed culture medium. The culture condition is that the temperature is 34 °C, the pH value is 6.5, and the culture time is 7-8 days. When the culture solution turns pale yellow and small bubbles rise, and the bacteria are in a vigorous metabolic period, expand the culture.

[0054] Cultivation of caproic acid bacteria liquid for production: Rinse the 10L earthenware pot with water → fermented grain filtrate, sodium acetate and ammonium sulfate (the amount added is half of the container) → add steam and heat to boil, keep for 30 minutes → wait for the altar to cool to 40 ℃, add high-temperature sterilized calcium carbo...

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Abstract

The invention relates to pit mud functional bacteria and a preparation method thereof, and belongs to the technical field of microbial fermentation. The technical problem to be solved is to provide the method for preparing the pit mud functional bacteria. The method for preparing the pit mud functional bacteria comprises the following steps of: a, preparing caproic acid bacterium solution, namely rejuvenating a caproic acid bacterium strain and performing amplification culture to obtain the caproic acid bacterium solution; b, preparing pit mud enriched bacterium solution, namely culturing strains in pit mud to obtain the pit mud enriched bacterium solution; and c, preparing the pit mud functional bacteria, namely mixing the caproic acid bacterium solution and the pit mud enriched bacterium solution, inoculating in a pit mud functional bacterium culture medium, and culturing for 22 to 27 days at the temperature of between 32 and 36 DEG C to obtain pit mud functional bacterium solution.

Description

technical field [0001] The invention relates to pit mud functional bacteria and a preparation method thereof, belonging to the technical field of microbial fermentation. Background technique [0002] The secret of good wine from old cellars lies in the rich functional flora of cellar mud in old cellars. Pit mud functional bacteria is not a microbiological classification, but a general term for microorganisms in pit mud that are beneficial to winemaking. Its dominant flora is caproic acid bacteria, butyric acid bacteria and other anaerobic bacillus, and its main function is to participate in the fermentation of the wine body to produce aroma. Among them, caproic acid bacteria are not only the most important functional bacteria for aroma production in Laojiao fermentation, but also functional indicator bacteria representing the production performance of Laojiao. However, in the production of Luzhou-flavor liquor, it was found that the fermented grains on the upper layer and ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/07
Inventor 张宿义方军易彬卢中明敖灵沈小娟
Owner LUZHOU PINCHUANG TECH CO LTD
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