A method for studying anticancer drugs by monitoring endocytosis in real time

An anti-cancer drug and real-time monitoring technology, applied in biochemical equipment and methods, microbial measurement/testing, fluorescence/phosphorescence, etc., can solve the problem of reduced reliability and inability to obtain comprehensive, dynamic and real-time visibility of endocytic transport data and other issues

Inactive Publication Date: 2011-11-30
INST OF PHYSICS - CHINESE ACAD OF SCI
View PDF1 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The current research strategy on cell endocytosis is to take pictures at different time points to obtain data, and find the effects of anticancer drugs through static pictures at several time points. Most of them are qualitative con

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for studying anticancer drugs by monitoring endocytosis in real time
  • A method for studying anticancer drugs by monitoring endocytosis in real time
  • A method for studying anticancer drugs by monitoring endocytosis in real time

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Method for real-time monitoring of endocytosis provided by the invention

[0026] In this embodiment, taking the anticancer drug paclitaxel as an example, the method for real-time monitoring of endocytosis provided by the present invention is described in detail, and the effect of paclitaxel on endocytosis is evaluated, as detailed below

[0027] Paclitaxel was first applied to the cells. The specific method is: paclitaxel (Invitrogen) was diluted with DMEM culture medium (Hyclone) to a concentration of 4 μM, and added to a culture dish growing human lung cancer cells (A549, purchased from Peking Union Medical College Cell Resource Center). And place it in a 37°C incubator for 2-4 hours.

[0028] Through the two-step labeling method, the cell membrane is connected to the EGF-QD probe. For details, see Jaiswal, J.K., H.Mattoussi, et al. "Long-term multiple color imaging of live cells using quantum dot bioconjugates." Nature Biotechnology 21, 47 -51 (2002), ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a method for real-time monitoring of cell endocytosis to study anticancer drugs. The method for real-time monitoring of the endocytosis process provided by the present invention comprises the following steps: 1) adding anticancer drugs to make it act on the cells to be tested; 2) connecting epidermal growth factor on the cell membrane of the cells to be tested by a two-step labeling method -Quantum dot probe; 3) Using a fluorescence microscope to observe and photograph under the condition that the light intensity is less than 100 μW, and track the endocytosis process according to the quantum dot label; 4) Quantitatively analyze the photographed data to obtain a relative endocytosis curve. The method provided by the present invention achieves the study of drug effects by quantitatively analyzing the effect of anticancer drugs on endocytosis through real-time observation throughout the whole process, and also considers the influence of cell shape to standardize the endocytic transport data of different cells, completely It can realize horizontal comparison and provide a new quantitative research method for the research, development, screening and therapeutic effect of anticancer drugs.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular, the invention relates to a method for researching anticancer drugs by monitoring cell endocytosis in real time. Background technique [0002] With the rapid development of life science research, bioluminescent technology has been widely used in cellular immunology, molecular biology, oncology and other fields. Fluorescence technology has become one of the important means of life science research. However, traditional fluorescent probes such as green fluorescent protein (GFP), Alexa Fluro, Cy3, Cy5, etc. have some insurmountable shortcomings, such as different degrees of fluorescence quenching, unstable light intensity, and cannot be applied to long-term observation experiments . However, many life processes are long-term and continuous changes, so a fluorescent probe with good stability is needed. [0003] Quantum dots (quantum dots, QDs), also known as semiconductor nanocrystals, a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N21/64C12Q1/02
Inventor 李辉王鹏业窦硕星刘玉如
Owner INST OF PHYSICS - CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap