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Electrochemical preparation method of electrode modified by polypyrrole-chitosan-enzyme composite film
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A technology for modifying electrodes and chitosan, applied in scientific instruments, material analysis by electromagnetic means, measurement devices, etc., can solve the problems of long preparation cycle and complex preparation process, and achieve controllable enzyme immobilization, abundant sources, Thickness controllable effect
Inactive Publication Date: 2013-06-19
WUHAN INSTITUTE OF TECHNOLOGY
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Problems solved by technology
However, the preparation process of enzyme-modified electrodes in the prior art is generally complicated and the preparation cycle is long
Method used
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Embodiment 1
[0019] 1) chitosan is dissolved in 0.1mol / L nitric acid solution to obtain solution A, and the mass percentage concentration of chitosan in solution A is 0.5%;
[0020] 2) adding pyrrole monomer to solution A to obtain solution B, the concentration of pyrrole monomer in solution B is 0.1mol / L;
[0021] 3) Add peroxidase enzyme to solution B, sonicate for 10 minutes to obtain solution C, the concentration of peroxidase enzyme in solution C is 0.1mg / mL
[0022] 4) Place the gold electrode as the cathode in solution C, electrify for electrochemical synthesis, the cathode potential is -0.6V, the electrochemical synthesis time is 5 minutes, polypyrrole, chitosan and enzyme are attached to the surface of the cathode, and the poly Pyrrole-chitosan-enzyme composite membrane modified electrode.
Embodiment 2
[0024] 1) chitosan is dissolved in 0.3mol / L nitric acid solution to obtain solution A, and the mass percentage concentration of chitosan in solution A is 1%;
[0025] 2) adding pyrrole monomer to solution A to obtain solution B, the concentration of pyrrole monomer in solution B is 0.2mol / L;
[0026] 3) Add glucose oxidase to solution B, sonicate for 20 minutes to obtain solution C, the concentration of glucose oxidase in solution C is 0.5 mg / mL;
[0027] 4) Put the platinum electrode as the cathode in the solution C, electrify for electrochemical synthesis, the cathode potential is -0.8V, the electrochemical synthesis time is 10 minutes, polypyrrole, chitosan and enzyme are attached to the surface of the cathode to obtain poly Pyrrole-chitosan-enzyme composite membrane modified electrode.
Embodiment 3
[0029] 1) chitosan is dissolved in 0.5mol / L nitric acid solution to obtain solution A, and the mass percentage concentration of chitosan in solution A is 2%;
[0030] 2) adding pyrrole monomer to solution A to obtain solution B, the concentration of pyrrole monomer in solution B is 0.3mol / L;
[0031] 3) Add lactate oxidase to solution B, sonicate for 15 minutes to obtain solution C, the concentration of lactate oxidase in solution C is 0.4 mg / mL;
[0032] 4) Put the glassy carbon electrode as the cathode in the solution C, electrify for electrochemical synthesis, the cathode potential is -1.2V, the electrochemical synthesis time is 10 minutes, polypyrrole, chitosan and enzyme are attached to the surface of the cathode to obtain Polypyrrole-chitosan-enzyme composite membrane modified electrode.
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Abstract
The invention relates to an electrochemical preparation method of an electrode modified by a polypyrrole-chitosan-enzyme composite film, and the preparation method comprises the following steps: 1) dissolving chitosan in a 0.01-1 mol / L nitric acid solution to obtain a solution A, wherein the mass percentage concentration of chitosan in the solution A is 0.5-5%; 2) adding a pyrrole monomer to the solution A to obtain a solution B, wherein the concentration of the pyrrole monomer in the solution B is 0.1-0.4 mol / L; 3) adding an enzyme to the solution B and ultrasonically treating for 10-30 minutes to obtain a solution C, wherein the concentration of the enzyme in the solution C is 0.01-1 mg / mL; and 4) placing a cathode in the solution C, electrifying for electrochemical synthesis so that polypyrrole, chitosan and the enzyme are attached to the surface of the cathode, thus obtaining the electrode modified by the polypyrrole-chitosan-enzyme composite film. The preparation method provided by the invention has the advantages of simple preparation conditions, easiness in operation, controllable thickness of the polypyrrole-chitosan-enzyme composite film and controllable enzyme immobilization amount.
Description
technical field [0001] The invention relates to a preparation method of a modified electrode, in particular to an electrochemical preparation method of a polypyrrole-chitosan-enzyme composite membrane modified electrode. Background technique [0002] Conductive polymer is a new research field developed in the 1970s. Due to its special structure and excellent physical and chemical properties, it has broad application prospects in electrode materials, modified electrodes, enzyme electrodes, and electromagnetic displays. Among them, the conductive polymer polypyrrole with conjugated double bonds is more and more popular due to its convenient synthesis and good oxidation resistance. Compared with other conductive polymers, it has the advantages of high conductivity, easy film formation, softness, and non-toxicity People pay attention. Polypyrrole-modified enzyme electrodes are a hotspot in biosensor research, and a variety of preparation methods have been developed. [0003] C...
Claims
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Application Information
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