Optical information analysis device and optical information analysis method

Abstract

An optical information analyzing device includes an irradiating unit that irradiates irradiation light to specimens, a transmitted light receiving unit that receives transmitted light and detects the transmitted light as a transmitted light signal, a scattering / fluorescent light receiving unit that receives lateral scattering light and fluorescent light and detects the lateral scattering light and the fluorescent light as a scattering / fluorescent light signal, a nozzle position adjusting mechanism, and an analyzing unit that measures the optical information on the specimen on the basis of the detected transmitted light signal and the detected scattering / fluorescent light signal and analyzes the specimen.

Description

technical field [0001] The present invention relates to an optical information analysis device and an optical information analysis method. In particular, it relates to an optical information analysis device and an optical information analysis method for measuring optical information of a sample by irradiating irradiation light on a sample to be measured that is dispersed in a liquid flowing in a flow channel. Background technique [0002] Proposals include the following devices (flow type position meter (flow type position meter), cell sorting instrument) in which a liquid containing biological particles (microscopic objects to be tested: specimen) that has been stained is placed in a flow path The flow channel forming the member (fluid cell) flows, the light irradiated from the illuminating unit (irradiating unit) contacts the biological particles, and the scattered light from the biological particles is detected by the detecting unit (light receiving unit). and fluorescen...

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Problems solved by technology

[0005] However, in the above-mentioned conventional device, there is a problem that when the sample flow passes through a position deviated from the center of the flow path in the flow cell, the optical axis of the irradiating part coincides with the sample flow. The optical axis of the optical system of the part does not cross the optical axis of the sample flow and the irradiation part

Therefore, the light receiving unit is configured to receive light in a wide range. As a result, the sensitivity of detected scattered light and fluorescence decreases, or the obtained optical information of the specimen varies.

[0006] In addition, there is a problem that the optical axis of the irradiation part cannot be adjusted and the optical axis of the irradiation part cannot be adjusted easily because the sample flow is actually produced only by flowing the liquid in the flow channel in the flow cell.

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