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A method for processing bovine somatic cell cloned embryos based on somatic cell nuclear transfer

A technology of somatic cell nuclear transfer and processing method, which is applied in the field of animal somatic cell cloning, can solve the problems of reprogramming failure, incompleteness, low cloning efficiency and reprogramming, and achieve the effect of improving development rate and quality, and efficient production

Inactive Publication Date: 2011-12-28
NORTHWEST A & F UNIV
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Problems solved by technology

[0003] At present, it is believed that the main reason for the low cloning efficiency is that the nucleus of the donor somatic cell has not been completely reprogrammed by the cytoplasm of the recipient ooplasm, that is, the reprogramming failure or incomplete
No changes in gene sequence are involved in this reprogramming process, mainly changes in epigenetic modifications

Method used

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  • A method for processing bovine somatic cell cloned embryos based on somatic cell nuclear transfer
  • A method for processing bovine somatic cell cloned embryos based on somatic cell nuclear transfer
  • A method for processing bovine somatic cell cloned embryos based on somatic cell nuclear transfer

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Embodiment Construction

[0027] In the method for processing bovine somatic cell cloned embryos constructed based on somatic cell nuclear transfer provided by the present invention, after the reconstruction body is constructed, the reconstruction body is treated with Oxamflatin to inhibit dehistone acetylase, which can significantly improve bovine somatic cell Developmental rate and quality of cloned blastocysts for efficient in vitro production of bovine somatic cell cloned embryos. The present invention will be further described in detail in conjunction with the specific operation process and comparative analysis results below, which is an explanation of the present invention rather than a limitation.

[0028] First give the source or preparation of the following reagents and culture / treatment solutions:

[0029] Oxamflatin, DMSO, trypsin, EDTA, penicillin, streptomycin, inorganic salts, and paraffin oil are products of Sigma; DMEM / F12 liquid medium and special-grade fetal bovine serum (FBS) are pro...

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Abstract

The invention discloses a method for processing bovine somatic cell cloned embryos constructed on the basis of somatic cell nuclear transplantation, which comprises the following steps of: injecting bovine somatic cells to be transplanted into denucleated oocyte to carry out electrofusion; and an hour later after the electrofusion is completed, selecting the successfully fused bovine somatic cellcloned embryos and processing the bovine somatic cell cloned embryos for 12 hours by 1muM of Oxamflatin. By the method, the developmental rate and the quality of the bovine somatic cell cloned embryos can be obviously improved and the bovine somatic cell cloned embryos can be efficiently produced in vitro.

Description

technical field [0001] The invention belongs to the technical field of animal somatic cell cloning, and relates to a method for processing bovine somatic cell cloned embryos constructed based on somatic cell nuclear transfer. Background technique [0002] Somatic cell cloning is a technology with great research and commercial value, which can be applied to therapeutic cloning, disease models, human organ transplantation, animal transgenic research, protection of endangered animal breeds, expansion of excellent livestock breeds, etc. But so far the efficiency of somatic cell cloning is still not high, which significantly restricts the wide application of this technology. [0003] At present, it is believed that the main reason for the low cloning efficiency is that the nucleus of the donor somatic cell is not completely reprogrammed by the cytoplasm of the recipient ooplasm, that is, the reprogramming fails or is incomplete. No changes in gene sequence are involved in this r...

Claims

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Application Information

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IPC IPC(8): C12N15/877
Inventor 苏建民张涌王勇胜
Owner NORTHWEST A & F UNIV
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