Differential stepwise centrifugal separation and detection method for large and small rubber particles of rubber trees

Abstract

The invention relates to a differential stepwise centrifugal separation and detection method for large and small rubber particles of rubber trees. In the method, fresh latex of the rubber trees is taken as a material, and the large and small rubber particles are separated under the action of different centrifugal forces through differential stepwise centrifugation of a centrifuge according to mass difference of the large and small rubber particles. The method comprises the following steps of: centrifuging the latex at a rate of 16,000rpm for 60 minutes, respectively transferring the upper large rubber particle part and a small rubber particle-containing serum part into clean centrifuge tubes, adding an isotonic solution (10mM Tris and 250mM sucrose) in a volume which is 3 to 5 times that of the upper large rubber particle part, regulating the pH value to be 7.0 by using HCl, and making the rubber particles fully suspended; and improving the centrifugal speed to 18,000rpm or reducing the centrifugal speed to 12,000rpm to fully separate the large and small rubber particles. The invention has the advantages that: the method mainly adopts centrifugation and is simple and feasible, and the large and small rubber particles of the rubber trees, which are completely separated and purified, can be quickly and efficiently obtained; moreover, the invention provides a scanning electron microscope detection method for identifying the separated and purified large and small rubber particles.

Description

technical field [0001] The invention relates to a differential speed step-by-step centrifugation and detection method of rubber particles of rubber tree size. Background technique [0002] 1. The basic situation of rubber latex rubber particles [0003] 1. Rubber particles are the gelatinous organelles of latex duct cells of the rubber tree [0004] Rubber Particle is a special organelle in Hevea brasiliensis Muell.Arg., which accounts for about 30-45% of the latex of Hevea brasiliensis Muell.Arg., and its main biological function is to carry out rubber biosynthesis. As a special organelle with rubber biosynthesis ability, rubber particles have great differences and certain similarities with other organelles such as mitochondria and chloroplasts. Under the electron microscope, it can be observed that the fully developed rubber particles are spherical, with an average diameter of 1-2 μm. The interior is a highly hydrophobic environment, and the main component is rubber hydr...

AI Technical Summary

Problems solved by technology

[0008] 3. Differences in the rubber biosynthesis rate of large and small rubber particles

Although molecular sieves and column chromatography have been tried in the past to separate large and small rubber particles in Hevea latex, it is difficult to obtain purified large and small rubber particles because the rubber particles are easy to solidify in the separation process of these methods. Particle separation and purification methods have not been effectively resolved