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Novel high-efficiency biocontrol bacterial fertilizer and production technology thereof

A new type of biocontrol technology, applied in biocides, bactericides, biocides, etc., can solve problems such as inability to carry out crop rotation and fallow, shortage of cultivated land resources, and many soil pathogens, and achieve good market prospects.

Inactive Publication Date: 2012-02-08
杨凌绿都生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the other hand, my country's arable land resources are in short supply, and a single crop has been planted for a long time, and it is impossible to carry out crop rotation and fallow, which makes soil pathogenic bacteria accumulate more and more.

Method used

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  • Novel high-efficiency biocontrol bacterial fertilizer and production technology thereof
  • Novel high-efficiency biocontrol bacterial fertilizer and production technology thereof
  • Novel high-efficiency biocontrol bacterial fertilizer and production technology thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] (1) Separation:

[0053] a. Collection and processing of soil samples: 20 parts of soil were collected from different locations in Qinling Mountains, Shaanxi Province, each weighed 3g, and placed in a triangular flask filled with 12mL of sterile distilled water. , centrifuged at 1500r / min for 15min, and the supernatant was taken to obtain the bacterial suspension. Using the gradient dilution separation method, spore suspensions of different dilutions were spread on 20 LB agar plates, and cultured in a 30°C incubator until a single colony grew.

[0054] b. Bacteria picking and purification: According to the morphological characteristics of Bacillus colonies, select several colonies with significantly different colony shapes, use sterile inoculation loops to streak and inoculate on LB agar plates, and culture them in a 30°C incubator until a single colony grows. colony. Then pick a single colony to streak and inoculate it on the sporulation medium, and cultivate it in a...

Embodiment 2

[0067] Example 2 Preparation of Carrier Peat Soil in Biocontrol Bacteria Wettable Powder

[0068] (1) Preparation of carrier materials and stabilizer additives

[0069] Choose peat soil, which is non-toxic to Bacillus strains, has good water absorption and water retention, pH value between 6.5 and 7.0, small particles, easy to stick to seeds, not easy to agglomerate, and cheap as the inoculation carrier. The peat soil is dried and pulverized, sieved with 100 sieve holes, placed in an autoclave at 100-121° C., sterilized continuously for 30-40 minutes, and then set aside.

Embodiment 3

[0070] Embodiment 3 Preparation of biocontrol bacteria wettable powder solid bacterial fertilizer

[0071] Get the bacillus liquid bacterial fertilizer suspension that prepares in embodiment 1, select the peat soil that handles well, and stabilizer, auxiliary agent, trace element liquid

[0072] Table 1 The proportioning composition table of other auxiliary raw materials of biocontrol bacterial fertilizer wettable powder of the present invention

[0073]

[0074] In the above composition ratio, each part can be measured in g or kg, or can be measured in other weight units, such as mg, t, etc. When preparing, the following are measured in g.

[0075] Take the bacillus liquid bacterial fertilizer suspension, the number of viable bacteria in the suspension is 2.0×10 10 pcs / ml to 3.0×10 10 1 / ml, according to 1:10 (v / w, ml / g), inoculate the fermented bacteria suspension on the peat soil after high temperature sterilization and mix evenly, then add stabilizer and auxiliary agen...

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Abstract

The invention relates to novel high-efficiency biocontrol bacterial fertilizer and a production technology thereof. The novel high-efficiency biocontrol bacterial fertilizer is named as Lvdu I. The novel high-efficiency biocontrol bacterial fertilizer is an active microbe-containing specific preparation prepared through activity recovery, propagation and expansion culture of strains, preparation of liquid bacterial fertilizer, preparation of solid bacterial fertilizer, and the like. The novel high-efficiency biocontrol bacterial fertilizer can treat multiple soil-borne diseases such as cucumber fusarium wilt, phytophthora capsici leonian, tomato bacterial wilt and the like. The novel high-efficiency biocontrol bacterial fertilizer named as Lvdu I can improve soil fertility, prolong fertilizer action time, promote growth of a few of crops, increase output, produce biocontrol effects on harmful microbes, and reduce crop diseases. The novel high-efficiency biocontrol bacterial fertilizer named as Lvdu I has the advantages of low costs, convenience of use, simple process flow, and no pollution and toxic residues.

Description

technical field [0001] The invention relates to a biocontrol bacterial fertilizer, in particular to a biocontrol bacterial fertilizer containing Bacillus subtilis isolated from soil, which can effectively treat various soil-borne diseases and promote plant growth. Fat. Background technique [0002] China feeds more than 20% of the world's population with 7% of its arable land, creating a miracle of achieving basic food self-sufficiency in a country with a lot of people and little land. However, the production of food crops, fruits and vegetables in our country has been constantly attacked by various microbial diseases, which has caused huge agricultural economic losses and has become one of the serious problems hindering the development of my country's agricultural economy. According to statistics, in recent years, the average annual occurrence area of ​​crop pests in my country has exceeded 100 million hectares, an increase of 41% compared with the 1980s; the annual damage...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00A01N63/00A01P3/00
Inventor 杨长锁杨轩
Owner 杨凌绿都生物科技有限公司
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