New method for positioning and cloning rice genes

Abstract

The invention discloses a new method for positioning and cloning rice genes. The method comprises the following steps of: 1, constructing a gene positioning colony; 2, constructing the gene positioning colony, a mapping colony and a gene effect colony; 3, determining a target gene area by using traits as guidance and using interval mapping as a basic method; 4, identifying target genes according to the 'Match' principle; 5, establishing a plurality of colonies for identifying gene effect; and 6, alternating genes when the genetic distance between the closely linked markers and genes is 0.1 to 1 centimeter, and performing functional complementation assay, wherein at the 'genome scanning' stage, the assay consumption is reduced, the mapping efficiency is improved, and the time interval for positioning the genes is shortened. Big 'secondary colonies' are easily generated, and target genes are 'approached' more easily. Because the basic colony is constructed and has high polymorphism and many target genes, if a new positioning colony needs to be constructed, the new colony can be derived or reconstructed. The method is suitable for positioning and cloning multiple genes. The method is favorable for revealing the gene action of certain specific traits and cloning one or more genes.

Description

technical field [0001] The invention belongs to a biological gene positional cloning method, in particular to a new method for rice gene positional cloning. Background technique [0002] Rice is the most important food crop and model plant. In 1991, Japan launched the "Rice Genome Program (RGP)", which was successively joined by the United States, China and other countries and regions, and became a global scientific project. Meanwhile, China announced in August 1992 that it would independently launch its own rice genome project. During the 20 years from 1991 to 2011, rice genetic maps, physical maps, transcription maps, and sequence maps were successively drawn. The greatest impact of RGP on agriculture and crop improvement is the positioning and cloning of genes, which has led to Breeding by design. After positioning genes (or QTL), varieties can be improved through aggregate breeding or molecular marker-assisted selection (MAS). Cloning genes can help breeders understan...

AI Technical Summary

Problems solved by technology

Its main problems and shortcomings are: (1) It is difficult to identify or clone the gene when the gene effect is small; (2) It is difficult to implement this method when the polymorphism in the target gene region is low or no polymorphism; (3) In the Pinpointing or cloning genes with this strategy is difficult when several or more genes act as haplotypes

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