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Primary amino radical tester

A measuring instrument and the technology of primary amino group, applied in the field of chemical analysis and testing instruments and equipment, can solve the problems of serious environmental pollution, great influence, inability to perform measurement, etc., and achieve the effects of keeping the environment clean, stable measurement data, and short detection period.

Inactive Publication Date: 2012-03-28
QILU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The test is greatly affected by the surrounding environment, and it cannot be measured at low temperatures
Paradigm Amino Acid Analyzer does not conduct unified treatment of waste gas and waste liquid, which seriously pollutes the environment

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0031] The primary amino acid measuring instrument of the present invention is composed of a reaction device (I), a gas measuring device (II), a gas washing device (III), and a recovery device (IV).

[0032] Preferably, the reaction device (I) includes a reactor (1), a first feeding pipe (2), a second feeding pipe (3) and a tee connecting pipe (14). Wherein the reactor contains a water bath jacket (4), and the outer wall of the water bath jacket (4) contains two interfaces (6) for connecting a circulating water bath. Preferably, the bottom of the reactor is a flat bottom, containing a first narrow-necked glass tube (7), a second narrow-necked glass tube (8) and a downward discharge tube (11). One end of the first thin-necked glass tube (7) is connected to the first feed pipe (2), and the other end passes through the water bath jacket (4) and is connected with the inner wall of the reactor (5) for adding the reaction solution; One end of the neck glass tube (8) is connected to...

Embodiment 2

[0038] 1. Experimental preparation:

[0039] When the present invention is used, the preparatory work is first carried out. At first, the gas washing liquid (20-40 g of potassium permanganate, 20-40 g of sodium hydroxide, 200-400 ml of deionized water) will be prepared, and then each piston is wiped with vacuum grease, and the reaction The upper port (12) of the device is wrapped with a sealing film and fixed with clips, the reactor drain pipe (11) and the gas washing hemispherical drain pipe (25) are clamped with water-stop clips, and the reactor jacket (4) communicates with the circulating water bath . The three-way connecting pipe cock (15) is screwed into The trachea cock (19) is turned into "\\" (such as Figure 1 The middle measuring air pipe cock (19)) ensures that the measuring air pipe (20) communicates with the recovery device (IV) by the three-way connection pipe exhaust pipe (11). Inject deionized water from the level ball (21), raise the level ball (21) (highe...

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Abstract

The invention discloses a primary amino radical tester, which is obtained by improving a paradigm amino acid tester, is mainly used for measuring the primary amino radical content of proteins, and is popularized and application to the measurement of the primary amino radical content of various organic compounds. The tester mainly comprises a reaction part, a gas measuring part, a gas washing part and a recovering part, and has the characteristics of rapidness, accuracy, cleanliness and the like.

Description

Technical field: [0001] The invention belongs to the field of chemical analysis testing instruments and equipment, and relates to an instrument mainly used for measuring the content of primary amino groups in proteins (including collagen polypeptides and various amino acids)—a primary amino group measuring instrument. Background technique: [0002] The methods currently used to measure the content of primary amino groups in proteins include ninhydrin colorimetry, TNBS colorimetry, micellar electrokinetic chromatography, formaldehyde method, Van Slyck method, and gas chromatography. Among them, ninhydrin colorimetry and TNBS colorimetry belong to spectrophotometric methods, and both have instrument errors. In addition, in the ninhydrin colorimetric method, the length of time for heating the color and the depth of the color after color development will cause errors; the TNBS colorimetric method is very specific, mainly reacting with the ε-amino group of lysine, and cannot Qua...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
Inventor 李天铎唐晓龙姜青伟许静
Owner QILU UNIV OF TECH
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