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Crosslinking-technology-based bionic bone interface, and construction method and application thereof

A technology of bionic interface and technology, applied in the field of bionic interface, can solve the problems of low absorption efficiency, desorption, etc., and achieve the effect of mild reaction conditions

Inactive Publication Date: 2012-05-02
THE SECOND AFFILIATED HOSPITAL ARMY MEDICAL UNIV
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Problems solved by technology

Second, to solve the problems of low absorption efficiency and desorption under certain circumstances in the traditional coating technology, the present invention utilizes covalent reaction to achieve a stable connection between the signal protein and the scaffold material, and improves the distribution strength of the biological ligand at the interface , to construct a surface biomimetic microenvironment with good tissue compatibility

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  • Crosslinking-technology-based bionic bone interface, and construction method and application thereof
  • Crosslinking-technology-based bionic bone interface, and construction method and application thereof
  • Crosslinking-technology-based bionic bone interface, and construction method and application thereof

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Embodiment Construction

[0029] The construction process of the present invention is described in detail below in conjunction with embodiment:

[0030] 1. CDH-11-EC 1-2 / FN III 7-10 - Assembly of the pBCP biomimetic interface, see figure 1 :

[0031] Using the method of homobifunctional cross-linking, the fusion protein CDH-11-EC 1-2 / FN III 7-10 Fixed to the material interface by covalent bonding. The homobifunctional crosslinking agent used is DTBP (C 8 h 18 N 2 o 2 S 2 Cl 2 ), the substance belongs to imidate, has water solubility, can permeate the membrane, and the two ends of the spacer arm (spacer arm) can form a bridge-like structure between the two molecules through the specific reaction with the amino group. Commonly used reagents for protein ligation reactions. The brief steps of the bionic interface construction are as follows,

[0032] (1). Blocking: BCP is placed in 85°C heat-denatured 1% BSA solution and soaked overnight (4°C) to block the non-specific binding sites on the s...

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Abstract

The invention relates to a crosslinking-technology-based bionic bone interface, and a construction method and application thereof. In the invention, biology signal-recombinant fibronectin / cadherin and a calcium phosphate ceramic micropore interface are subjected to covalent coupling. The invention aims to promote the high-efficiency adhesion and targeted differentiation of cells on the interface and belongs to the field of interface tissue engineering. The signal protein is recombinant protein of fibronectin type III (FNIII)7-10 carrying Pro-His-Ser-Arg-Asn (PHSRN) and Arg-Gly-Asp (RGD) motifs and cadherin functional domain EC1-2. crosslinking molecules are same bifunctional crosslinking agent ditertiary butyl peroxide (DTBP), and protein and materials can be bridged through amino acetylation. Plantation density and mineralization capacity of seed cells on the interface are improved through isophil and heterophilic adhesion of the signal protein and integrin subtype mediated osteoblasts induction. The interface characteristics are identified in the aspects of appearance topology, surface chemistry, cytocompatibility and the like during implementation. The crosslinking-technology-based bionic bone interface is suitable for preparation of alternative materials of bioactivity tissue engineering of compound cells.

Description

technical field [0001] The invention relates to a bionic interface for coupling biological signal recombinant fibronectin / cadherin and biphasic calcium-phosphorus ceramics through homobifunctional cross-linking technology. It belongs to the field of interface tissue engineering. Background technique [0002] In order to make bone repair materials have good bioactivity and tissue compatibility, they can be modified according to the principles of bionics, that is, to immobilize bioactive molecules on the surface of artificial synthetic materials or natural materials without changing the properties of the matrix. Tissue engineering materials with specific biological and physical and chemical properties transform the "inert" interface into a biological interface that is conducive to providing adhesion, proliferation and differentiation signals for seed cells. [0003] The specific adhesion of cells on the substrate surface is mainly determined by the adsorbed protein molecular ...

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Application Information

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IPC IPC(8): A61L27/54A61L27/34A61L27/12
Inventor 张瑗张峡朱洁初同伟周跃
Owner THE SECOND AFFILIATED HOSPITAL ARMY MEDICAL UNIV
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