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Method for separating and purifying eupatorium odoratum flavonoid substance by using HZ816 macroporous adsorption resin

A HZ816, resin separation technology, applied in the field of green chemical technology, can solve the problems of poor safety, product quality and market competitiveness, etc., and achieve the effect of operation and product safety, novel theory and environmental friendliness

Inactive Publication Date: 2012-10-10
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The above-mentioned extraction, separation and purification method related to the plane grass can prepare the plane grass flavonoids and its monomers with a certain purity and yield, but due to the limitation of technical means and the use of a large amount of toxic organic solvents or chemical preparations in the process of processing , so that the product obviously has defects such as poor safety, product quality and insufficient market competitiveness; at the same time, the use of ultrasonic-assisted extraction and macroporous adsorption resin to separate and purify aircraft grass flavonoids has not yet been reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Dry and pulverize plane grass at 60°C, use edible ethanol with a volume concentration of 50%, extract 1 g of plane grass powder with 20ml of edible ethanol at 25°C with 160W ultrasonic wave for 1 hour, pass through 200-mesh nylon cloth, Centrifuge with a 4000r / min centrifuge for 10min, take the supernatant; concentrate the supernatant at -0.07MPa, 60°C, recover the edible ethanol solvent, and dry it in vacuum to obtain the crude extract, which is colorimetric with aluminum trichloride method, the purity of flavonoids was measured to be 9.15%.

[0020] The pretreated HZ816 macroporous adsorption resin was selected as the separation and purification resin of the flavonoids of the plane grass, and the above-mentioned flavonoids crude extract was formulated into a solution with a flavonoid concentration of 3 mg / mL; at room temperature, 8BV of this solution was mixed with At a flow rate of 3BV / h, pass through a chromatographic column equipped with HZ816 macroporous adsorptio...

Embodiment 2

[0022] Dry and pulverize plane grass at 50°C, use edible ethanol with a volume concentration of 60%, according to the ratio of 1g plane grass powder to 25ml edible ethanol, extract with 200W ultrasonic wave at 20°C for 1.5h, and pass through 200 mesh nylon cloth , and centrifuged with a 4500r / min centrifuge for 5min, the supernatant was taken; the supernatant was concentrated at -0.05MPa and 50°C, the edible ethanol solvent was recovered, and the crude extract was obtained by using aluminum trichloride ratio Color method, the measured purity of flavonoids was 8.87%.

[0023] The pretreated HZ816 macroporous adsorption resin was selected as the resin for the separation and purification of flavonoids from the flavonoids, and the crude extract of the flavonoids from the above flavonoids was prepared into a solution with a concentration of flavonoids of 2.75 mg / mL. At room temperature, pass 7BV of the solution through a chromatographic column equipped with HZ816 macroporous adsorp...

Embodiment 3

[0025] Dry and pulverize plane grass at 55°C, use edible ethanol with a volume concentration of 50%, according to the ratio of 1g plane grass powder to 15ml edible ethanol, extract with 120W ultrasonic wave at 22°C for 2 hours, pass through 200-mesh nylon cloth, Centrifuge with a 3500r / min centrifuge for 15min, take the supernatant; concentrate the supernatant at -0.09MPa, 55°C, recover the edible ethanol solvent, and dry it in vacuum to obtain the crude extract, which is colorimetric with aluminum trichloride method, the purity of flavonoids was measured to be 8.99%.

[0026]The pretreated HZ816 macroporous adsorption resin was selected as the resin for the separation and purification of flavonoids from the plant grass, and the crude extract of the flavonoids from the plant plant was prepared into a solution with a concentration of flavonoids of 3.25 mg / mL. At room temperature, pass 9BV of the solution through a chromatographic column equipped with HZ816 macroporous adsorptio...

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Abstract

The invention discloses a method used for separating and purifying a eupatorium odoratum flavonoid substance by using HZ816 macroporous adsorption resin. According to the invention, the flavonoid substance is extracted from eupatorium odoratum by using edible ethanol, such that a crude extract is obtained; the crude extract is separated and purified by using HZ816 macroporous adsorption resin, such that an extract of an eupatorium odoratum flavonoid substance is obtained. With the method provided by the invention, the eupatorium odoratum flavonoid substance is safely extracted and separated with no toxicity by using edible ethanol, such that a harmful matter is turned into a valuable matter. With the method provided by the invention, safe and effective eupatorium odoratum flavonoid substance extracts with different purity specifications can be industrially produced. With the method, eupatorium odoratum resources can be safely, conveniently, reasonably, and economically utilized with higher values, and industrialized productions of product series can be actually realized. With the method, the concept of green chemical production and the strategy of virtuous circular economy can be realized.

Description

Technical field: [0001] The invention relates to a green chemical process technology for separating and purifying flavonoids from plane grass, in particular to a green separation and purification method for separating and purifying flavonoids in plane grass by using HZ816 macroporous adsorption resin. Background technique: [0002] Airplane grass (Eupatorium odoratum L.), Compositae (Compositae) Eupatorium (Eupatorium), also known as Eupatorium, perennial herb, aircraft grassland produced in Central America, now widely distributed in South America, Africa, tropical Asia, born in Hillsides, fields, and roadsides are mainly distributed in Taiwan, Guangdong, Hong Kong, Hainan, Guangxi, Yunnan, and southwestern Guizhou in China. [0003] Airplane grass has become a worldwide vicious weed. It is an alien plant that invades our country. It is easy to reproduce and grows fast. It invades farmland, destroys other vegetation, affects the normal growth of plants, and poses a serious t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/28A61P29/00A61P7/04A61P17/00A61P31/04A61P17/02
Inventor 肖文军盛达成谭支良龚志华
Owner HUNAN AGRICULTURAL UNIV
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