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Method for separating and purifying blueberry anthocyanidin by using HP2MGL macroporous resin

A blueberry anthocyanin, separation and purification technology, applied in organic chemistry and other directions, can solve the problems of insufficient product quality and market competitiveness, low purity and yield, and high equipment cost, and achieves operation and product safety, environmental friendliness, and technology. effect of science

Inactive Publication Date: 2012-07-04
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Obviously, although the above-mentioned extraction, separation and purification methods related to blueberry fruit anthocyanins can prepare blueberry anthocyanin extracts with certain purity and yield, due to the limitation of technical means and the use of a large amount of toxic organic solvents or chemical preparations in the process , so that the product obviously has defects such as poor safety or low purity and yield, high equipment cost, and insufficient product quality and market competitiveness.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The cleaned HP2MGL macroporous adsorption resin was selected as the separation and purification resin of blueberry anthocyanins, and the crude blueberry anthocyanin extract was prepared with 75% volume concentration and pH=2.0 acidified edible ethanol to prepare a solution with a concentration of 0.75mg / mL . At room temperature, the 4.0BV solution was adsorbed and separated through a separation column equipped with HP2MGL macroporous resin at a flow rate of 2BV / h until the adsorption was saturated, and then washed with 4.0BV of distilled water at a flow rate of 1BV / h until it became colorless, and then Elute the anthocyanins with 5.5BV volume concentration of 50% edible ethanol at a flow rate of 2.0BV / h, collect the eluate, concentrate the eluate at -0.05MPa, 45℃, recover the edible ethanol, and freeze in vacuum After drying, 3.35 g of purified blueberry anthocyanin extract was obtained. Using pH difference method, the anthocyanin yield was 6.41%, the purity was 7.01%, a...

Embodiment 2

[0025] The cleaned HP2MGL macroporous adsorption resin is selected as the separation and purification resin of blueberry anthocyanins, and the crude blueberry extract is prepared into a solution with a concentration of 0.8 mg / mL with acidified edible ethanol with a volume concentration of 85% and pH=3.0. At room temperature, the 5.0BV solution was passed through a separation column equipped with HP2MGL macroporous resin at a flow rate of 3BV / h for adsorption and separation until the adsorption was saturated, and then washed with 6.0BV of distilled water at a flow rate of 2BV / h until it became colorless, and then Elute anthocyanins with 6.2BV 55% edible ethanol at a flow rate of 3.0BV / h, collect the eluate, concentrate the eluate at -0.07MPa, 50℃, recover the edible ethanol, and freeze in vacuum After drying, 3.05 g of purified blueberry anthocyanin extract was obtained. The anthocyanin yield was 6.96%, the purity was 7.20%, and the anthocyanin recovery rate was 94.46% as measure...

Embodiment 3

[0027] The cleaned HP2MGL macroporous adsorption resin is selected as the blueberry anthocyanin separation and purification resin, and the crude blueberry extract is prepared with acidified edible ethanol with a volume concentration of 95% and pH=4.0 to a liquid with a concentration of 0.90 mg / mL. At room temperature, the 6.0BV of this liquid was passed through a separation column equipped with HP2MGL macroporous resin at a flow rate of 3.5BV / h for adsorption and separation until the adsorption was saturated, and then washed with 8.0BV of distilled water at a flow rate of 3BV / h until it became colorless. Then eluted anthocyanins with 7.5BV of 60% edible ethanol at a flow rate of 3.5BV / h, collected the eluate, concentrated the eluate at -0.09MPa, 55℃, and recovered the edible ethanol. After freeze-drying, 3.43 grams of purified blueberry anthocyanin extract was obtained. The anthocyanin yield was 5.88%, the purity was 7.30%, and the anthocyanin recovery rate was 95.18% as measure...

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Abstract

The invention discloses a method for separating and purifying blueberry anthocyanidin by using HP2MGL macroporous resin, which comprises: extracting blueberry anthocyanidin coarse extract from blueberry by acidified edible ethanol; and separating and purifying blueberry anthocyanidin coarse extract by using HP2MGL macroporous resin to obtain an anthocyanidin extract. In the method, edible ethanolis used to extract and separate blueberry anthocyanidin safely without toxin, safe and effective blueberry anthocyanidin extract at different concentrations can be produced in industry, the safe, simple, convenient, reasonable and economic high-value utilization of blueberry resources and industrial production of serious products are really realized, and the philosophy of green chemical production and policy of circular economy are fully demonstrated.

Description

Technical field: [0001] This research involves a green chemical technology for separating and purifying anthocyanins from blueberries, especially a green separation and purification method for separating and purifying anthocyanins from blueberries using HP2MGL macroporous resin. Background technique: [0002] Blueberry, belonging to the azalea family (Ericaceae), lingonberry (Vacciniuml spp), perennial deciduous or evergreen shrubs. There are currently 450 species of bilberry in the world, and its wild species are widely distributed in the temperate and subtropical regions of the northern hemisphere, as well as tropical mountains in America and Asia. According to reports, there are currently 32 species of bilberry in my country, which are widely distributed in Changbai Mountain, Daxinganling, Liaodong Peninsula, Jiaodong Peninsula, Yangtze River Basin and South China and Southwest China, providing good natural advantages and resources for the development of bilberry. Advantage. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/62
Inventor 肖文军李勤龚志华张盛李安文
Owner HUNAN AGRICULTURAL UNIV
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