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Bio-enzyme-assisted extraction method for tea saponin

An auxiliary extraction and tea saponin technology, which is applied in the direction of sugar derivatives, sugar derivatives, sugar derivatives preparation, etc., can solve the problems of tea saponin dark color, complicated process, high production cost, etc.

Active Publication Date: 2012-05-09
FUJIAN SHENGHUA AGRI SCI & TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The water immersion method has low production cost and simple process, but the tea saponin is dark in color and low in purity; the organic solvent extraction of tea saponin is relatively light in color and high in purity, but the production cost is high and the process is complicated

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Add 100g of 40-mesh camellia oleifera meal into 300g of water, soak at 90°C for 2h, cool to 60°C; add 0.05g of cellulase and 0.075g of amylase, and catalyze the reaction at 50-60°C for 6h; add 300ml of 95% ethanol, and stir Evenly, let it stand for 1 hour, and filter to obtain the filtrate; after concentrating the filtrate and recovering ethanol, 50 g of the concentrated liquid is obtained, adding 150 g of ether, filtering, and recovering the ether from the filtrate; washing and drying the precipitate to obtain 11.5 g of tea soap with a purity of 94%. vegetarian products.

Embodiment 2

[0020] Add 100g of 40-mesh camellia oil into 250g of water, soak at 90°C for 2h, cool to 60°C; add 0.05g of cellulase, 0.075g of amylase, and 0.05g of pectinase, and catalyze the reaction at 50-60°C for 8h; 350ml of 95% ethanol, stir evenly, let stand for 1h, and filter to obtain the filtrate; after concentrating the filtrate to recover ethanol, obtain 50g of concentrated solution, add 150g of ether, filter, and recover ether from the filtrate; wash and dry the precipitate to obtain 11.8g of purity It is 94% tea saponin product.

Embodiment 3

[0022] Add 100g of 40-mesh camellia oleifera meal into 400g of water, soak at 90°C for 1.5h, cool to 50°C; add 0.15g of cellulase to catalyze the reaction at 50-60°C for 5h; add 300ml of 95% ethanol, stir evenly, and let stand for 1h , filtered to obtain the filtrate; after the filtrate was concentrated and reclaimed ethanol, 50g of the concentrated solution was obtained, and 200g of ether was added, filtered, and the ether was reclaimed from the filtrate; the precipitate was washed and dried to obtain 12.2g of tea saponin products with a purity of 90%.

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PUM

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Abstract

The invention discloses a bio-enzyme-assisted extraction method for a tea saponin, belonging to the method for extracting the tea saponin from camellia oleifera. The method comprises the following steps of: (1) adding water to the camellia oleifera to soak; (2) mixing a bio-enzyme and an extracted substance leaching solution, and performing a catalytic reaction; (3) with ethanol as a leaching agent, leaching the extracted substance subjected to the enzyme treatment, and extracting tea saponin components; (4) concentrating the extracted solution and precipitating by using diethyl ether; and (5) washing and drying the precipitates to obtain tea saponin products. The key technique of the invention is hydrolyzing sea saponin and polysaccharide composite by using bio-enzymes, so that the yielding rate and the purity of the tea saponin can be improved. The tea saponin prepared by using the method disclosed by the invention has the purity of more than 90% and the yielding rate of more than 85%. The method disclosed by the invention is simple, can be put into production by using traditional equipment and is suitable for mass production, and the additional value of the camellia oleifera isgreatly improved.

Description

technical field [0001] The invention relates to a method for extracting tea saponin from camellia oleifera dregs. Background technique [0002] Camellia oleifera is a unique woody oil plant in my country, and it is an oil crop vigorously developed in our country, and its comprehensive utilization value is very high. Tea saponin, also known as tea saponin, is a pentacyclic triterpenoid compound extracted from camellia oleifera. It consists of three parts: ligand, sugar body and organic acid. It is a natural non-ionic surfactant with excellent performance. It has strong foaming, emulsifying, dispersing, and moisturizing effects, and has physiological activities such as impermeability, anti-inflammatory, analgesic, and anti-cancer. It can be widely used in daily chemicals, building materials, textiles, pesticides, and some other fields. Its economic Higher value. [0003] At present, the extraction methods of tea saponin include water immersion method and organic solvent meth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00C07H15/256C07H1/08
Inventor 卢玉胜
Owner FUJIAN SHENGHUA AGRI SCI & TECH DEV