Manufacturing method of ceramide generation accelerator

A ceramide, a technology for a manufacturing method, applied in the directions of microorganism-based methods, biochemical equipment and methods, preparations for skin care, etc., to achieve the effect of improving moisturizing ability

Inactive Publication Date: 2012-05-23
KAO CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0013] However, there is no report on the preparation of ceramide production accelerators or glucosylceramide production accelerators using Pichia pastoris

Method used

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  • Manufacturing method of ceramide generation accelerator
  • Manufacturing method of ceramide generation accelerator

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049]

[0050] First, the culture medium is prepared according to the following method: Soak soybeans in water for 12 hours to allow the soybeans to fully absorb water, then grind and crush the absorbed soybeans, filter them to remove liquid components, and drain the remaining residues to obtain bean dregs. The okara was mixed with glucose and water, and the concentrations of the okara and glucose in the mixture were respectively 10w / v% and 1w / v%, and the mixture was used as a culture medium.

[0051] Then, add 100ml of the above-mentioned culture medium to the Erlenmeyer flask, inoculate a certain amount of Pichia anomala (preserved in CICIM, Jiangnan University Industrial Microbiology Resource and Information Center, No. 360-20-3), and inoculate at 30°C cultured for 3 days.

[0052] The resulting culture was then centrifuged (3000 rpm, 10 minutes). The remaining supernatant was diluted with 2.5 times of absolute ethanol, and 1ml of the mixture was taken as sample A of th...

Embodiment 2

[0070] In the preparation process of the ceramide / glucosylceramide production accelerator of Example 1, Pichia guilliermondii (preserved in CICIM, Jiangnan University Industrial Microbiology Resources and Information Center, No. 422-19-3) was used Instead of Pichia anomala (Pichia anomala), and adding 2.5 times of absolute ethanol to the supernatant obtained after bean dregs fermentation to dilute, the mixture was ultrasonically treated for 10 minutes, centrifuged (3000rpm) for 10 minutes, and the supernatant was collected ( About 70ml), the supernatant was filtered with filter paper to obtain the filtrate, and the filtrate was dried to constant weight in a vacuum drier, as samples B and C of the ceramide / glucosylceramide production accelerator of the present invention, the dried samples were dissolved in 50% ethanol , Samples B and C were prepared into 1% and 0.1% solutions respectively, and stored in a 4°C refrigerator as an additive for cell culture. Other than that, the sa...

Embodiment 3

[0073] In the preparation process of the ceramide / glucosylceramide production accelerator in Example 1, Pichia norvegensis (preserved in CICIM, Jiangnan University Industrial Microbiology Resource and Information Center, number CICIM Y0151) was used to replace Pichia anomaly (Pichia anomala), except that it was cultured in the same manner as in Example 1.

[0074] The resulting culture was then centrifuged (3000 rpm, 10 minutes). The remaining supernatant was diluted with 2.5 times absolute ethanol, and 1ml of the mixture was taken as sample D of the ceramide / glucosylceramide production accelerator of the present invention, and stored in a refrigerator at 4°C for use as an additive for cell culture.

[0075] The above-mentioned mixture diluted with absolute ethanol was ultrasonically treated for 10 minutes, centrifuged (3000rpm) for 10 minutes, and the supernatant (about 70ml) was collected. The supernatant was filtered with filter paper to obtain the filtrate, and the filtrat...

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Abstract

The invention provides a manufacturing method of a ceramide generation accelerator and/or a glucosylceramide generation accelerator, wherein Pichia microorganisms are cultured in a culture medium containing beam dregs and saccharides, and the ceramide generation accelerator and/or the glucosylceramide generation accelerator are/is obtained from an obtained supernatant. According to the manufacturing method disclosed by the invention, by utilization of easily obtainable substances as the culture medium and specific microorganisms, the ceramide generation accelerator and/or the glucosylceramide generation accelerator can be acquired at low cost and high efficiency.

Description

technical field [0001] The present invention relates to a method for producing a ceramide production promoter and / or a glucosylceramide production promoter capable of promoting the production of ceramide and / or glucosylceramide in the skin. Background technique [0002] The skin has the ability to produce ceramide and glucosylceramide. In the skin, especially in the stratum corneum of the epidermis, ceramide synthesized and secreted by epidermal cells is accumulated, which is the main component of intercellular lipids. The ceramide synthesized by epidermal cells is temporarily stored in the form of glucosylceramide or sphingomyelin; it is then excreted outside the cell, where glucocerebrosidase (glucocerebrosidase) and sphingomyelinase (sphingomyelinase) ) to form ceramide again, thereby functioning as an intercellular lipid. Ceramides and glucosylceramides in the skin have physiological effects of improving the moisturizing function of the skin, the barrier function of th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/02A61K8/99A61Q19/00C12R1/84
Inventor 孔凡旗
Owner KAO CORP
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