Platycodon grandiflorum polysaccharide, and degradation product, preparation method and application thereof
A technology of Platycodon grandiflorum polysaccharide and degradation products, which can be applied in the directions of sugar derivatives, sugar production, sugar derivatives, etc., and can solve the problems of drug resistance of anti-tumor drugs.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0030] Example 1 Extracting platycodon polysaccharides from platycodon rhizome
[0031] Take 3000 g of dried platycodon root, degrease 2 times (5 days / time) with 15 L of 95 wt % ethanol, filter and then dry naturally at room temperature. The dried bellflower decoction pieces were extracted with boiling water, adding 30 L of water each time for 6 hours, and extracted 4 to 6 times in total. After extraction, filtered to obtain the extract, the sulfuric acid-phenol method was used to detect the sugar content in the extract until the sugar reaction was not obvious. The extracts were combined, concentrated to about 10L, deproteinized with 15% (w / v) trichloroacetic acid, centrifuged, and the resulting supernatant was neutralized to pH 7.0 with 10% NaOH, and then dialyzed against flowing water for 48 hours, the inner liquid Concentrate to 1-2L, add three times the volume of 95% ethanol under stirring, let it stand overnight, and centrifuge, the obtained precipitate is washed with abs...
Embodiment 2
[0040] Example 2 Preparation of Platycodon grandiflora polysaccharide degradation product
[0041] Take 250mg of Platycodon grandiflora pectin polysaccharide and place it in a dry 150ml round bottom flask, add 50ml of 0.05mol / L trifluoroacetic acid, seal it tightly, and incubate at 100°C for 1h. After the reaction liquid was distilled off under reduced pressure to remove trifluoroacetic acid, it was dialyzed against distilled water (500ml×4) for 48h, and the inner and outer liquids of the dialysis bag were respectively concentrated and then freeze-dried. The inner solution was lyophilized to obtain secondary platycodon polysaccharide 4-3bde (98 mg). Combine the lyophilized products of the two dialyzed external fluids, separate them with Bio-Gel P-2 gel filtration column, detect them with sulfuric acid-phenol method, use standard sugar as a control, collect the components according to the peak position and freeze-dry them to obtain four components , respectively component 1 (4...
PUM
Property | Measurement | Unit |
---|---|---|
Specific rotation | aaaaa | aaaaa |
Average molecular weight | aaaaa | aaaaa |
Average molecular weight | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com