Essential oil preparation for preventing and treating tobacco mosaic virus and application thereof
A technology of tobacco mosaic virus and lemongrass essential oil, applied in application, disinfectant, biocide, etc., can solve the problems of anti-tobacco mosaic virus activity, which is rarely introduced, and avoid environmental pollution and pesticide residue problems, and planting areas Widespread, Inexpensive Effects
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Embodiment 1
[0020] Embodiment 1: the preparation of lemongrass essential oil and tangerine peel essential oil
[0021] Material: Lemongrass ( Cymbopogon citratus ) belongs to Gramineae (Gramineae) Citronella ( Cymbopogon ) green leafy herbs;
[0022] Tangerine peel is taken from citrus ( Citrus reticulata) peel of citrus ( Citrus reticulata) Rutaceae (Rutaceae) citrus genus ( Citrus )plant.
[0023] Both lemongrass and tangerine peel are commercially available.
[0024] (1) Lemongrass essential oil extraction: use scissors to cut lemongrass dried leaves into 2-3cm small pieces, accurately weigh 500g, put it into a steam distillation device with a volume of 5L, inject 3L of distilled water, and soak for 30min. Turn on the power supply, wait for the distillation bottle to boil for 60 minutes and then complete the distillation, collect the yellow oil on the liquid surface, and dry it with anhydrous sodium sulfate to obtain pure lemongrass essential oil. Put it in a brown bottle a...
Embodiment 2
[0028] Embodiment 2: Essential oil preparation and application for preventing and treating tobacco mosaic virus
[0029] 1. See 1 for the formula of the essential oil preparation for preventing and treating tobacco mosaic virus described in this embodiment.
[0030] 2, the preparation method of the essential oil preparation of preventing and treating tobacco mosaic virus described in the present embodiment:
[0031] Mix 2 parts of lemongrass essential oil, 4 parts of tangerine peel essential oil, 2 parts of Tween 20, and 8 parts of dimethyl sulfoxide according to the volume ratio to make emulsion, which is the essential oil preparation for preventing and treating tobacco mosaic virus. The lemongrass essential oil and tangerine peel essential oil described in the essential oil preparation are prepared according to the preparation method described in Example 1.
[0032] 3. The essential oil preparation for preventing and treating tobacco mosaic virus is used in passivation, tre...
specific Embodiment approach
[0033] The essential oil preparation for preventing and treating tobacco mosaic virus is diluted to 1000 times with water, and it is thoroughly mixed to form an emulsion of essential oil preparation for passivation, treatment and prevention experiments of tobacco mosaic virus. The specific implementation is as follows:
[0034] ① Preparation of tobacco mosaic virus suspension: Take 100g of tobacco leaves (variety K326) infected with tobacco mosaic virus disease, cut them into pieces, put them in a mortar, add a little quartz sand and 20ml of phosphate buffer (0.2M, pH5. 7-8.0), fully ground. Use a graduated cylinder to add phosphate buffer to make up to 100ml, and store in a refrigerator at 4°C for inoculation experiments.
[0035] The identification method of tobacco leaves infected with tobacco mosaic virus disease shall be sampled and identified according to "GB / T23222-2008 (Classification and Investigation Methods of Tobacco Diseases and Pests)". Tobacco mosaic virus in ...
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