Application of MaGCS protein in banana fruit maturation

Abstract

The invention discloses application of MaGCS protein in banana fruit maturation. The invention provides a new post-harvest fresh-keeping technology established on the basis of gene expression regulation. Specifically, the technology comprises: immersing harvested banana fruits in 100mM of an oxaloacetic acid solution for 2h so as to obtain banana fruits with at least one characteristic of the following 1)-2): 1) conducting immersion for 2h with 100mM of an oxaloacetic acid solution to promote MaGCS expression of the banana fruits; 2) carrying out immersion for 2h with 100mM of an oxaloacetic acid solution to promote maturation of the banana fruits; immersing harvested banana fruits in a citric acid solution with a concentration of 1%(w / v) for 1h so as to obtain banana fruits with at least one characteristic of the following 3)-4): conducting immersion for 1h with a citric acid solution with a concentration of 1%(w / v) so as to inhibit MaGCS expression of the banana fruits; and 4) carrying out immersion with a citric acid solution with a concentration of 1%(w / v) for 1h so as to inhibit maturation of the banana fruits. The amino acid sequence of the MaGCS protein is the sequence 2 of a sequence table. Experiments in the invention prove that, expression of MaGCS can be induced and inhibited respectively by oxaloacetic acid (OA) and citric acid (CA) so as to control the maturity change of harvested banana fruits. Application of the gene in the invention is of important theoretical and practical significance in establishing new technologies for controlling fruit maturation of harvested bananas and cultivating fine varieties.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of a MaGCS protein in banana fruit ripening. Background technique [0002] Banana is a typical climacteric fruit. The molecular biology research on the ripening mechanism of banana fruit will help to develop new technologies for postharvest preservation based on gene expression regulation and improve the in-depth research on fruit ripening regulation. These The research has important theoretical and practical significance in the development of banana industry. [0003] Ethylene is the core of banana postharvest ripening. Unlike most climacteric fruits, the increase and decrease of ethylene release rate in banana fruit during respiratory climacteric are drastic. Therefore, the regulation mechanism of ethylene biosynthesis in banana is considered May be different from other climactic fruits. Aminocyclopropane carboxylic acid (1-aminocyclopropane-1-carboxylic acid, ACC...

AI Technical Summary

Problems solved by technology

It is speculated that this gene may be related to ethylene biosynthesis in the postharvest ripening process of bananas, and may regulate or affect the postharvest ripening process of banana fruits

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