Application of MaGCS protein in banana fruit maturation
A banana and fruit technology, applied in the biological field, can solve problems affecting the ripening process of bananas, etc.
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[0032] The experimental methods used in the following examples are conventional methods unless otherwise specified.
[0033] The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.
[0034] 1. Source of materials
[0035] The Brazilian banana (M.AAA Group Cavendish) fruit was collected from the experimental base of the Institute of Tropical Biotechnology, Chinese Academy of Tropical Agricultural Sciences.
[0036] 2. Discovery of the MaGCS gene
[0037] A cDNA fragment of citrate synthase gene was obtained from the suppression subtractive hybridization library (SSH) in the early stage of banana fruit ripening, and the full length of the citrate synthase gene was cloned in banana fruit by RACE technology, named MaGCS, Its nucleotide sequence is sequence 1 in the sequence list. The amino acid sequence of the encoded protein is sequence 2 in the sequence list, and the protein is named MaGCS. Sequence 1 in t...
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