Uniform fluorescent microball and preparation method

Abstract

The invention discloses a uniform fluorescent microball and a preparation method. The preparation method includes the following steps: (1) preparing porous polymer microballs with uniform particle diameter and hole diameter in advance; (2) compounding quantum dots in the polymer microballs prepared in the step (1) so as to obtain quantum-dot polymer composite microballs; and (3) dispersing the quantum-dot polymer composite microballs prepared in the step (2) into ethanol/water mixed solution and adding ethyl silicate to prepare the fluorescent microball with the surface covered by silicon dioxide. The particle diameter of the fluorescent microball prepared by the above preparation method ranges from 500nm to 100 mu m and is uniform and controllable, and the fluorescent microball is high in fluorescent efficiency and stable in chemical property and has potential application value in the bioinstrumentation and medical fields.

Description

technical field [0001] The invention relates to the field of polymer materials, in particular to a fluorescent microsphere with uniform particle size and a preparation method thereof. Background technique [0002] Fluorescence is a kind of cold luminescence phenomenon of photoluminescence. When a substance at room temperature is irradiated by incident light of a certain wavelength (usually ultraviolet or X-ray), it enters an excited state after absorbing the light energy, and immediately de-excites and emits outgoing light with a wavelength longer than the incident light (usually with a wavelength of visible light) band), and once the incident light is stopped, the luminescence disappears immediately. The outgoing light with this property is called fluorescence. In recent years, as a new type of functional material, fluorescent microspheres have played an important role in biomedical fields such as fluorescent immunoassay, biosensor and high-throughput drug screening due t...

AI Technical Summary

Problems solved by technology

[0007] However, there are still many shortcomings in the fluorescent microspheres prepared at present, such as the particle size of the microspheres is very uneven, the fluorescence efficiency is low, and the fluorescent substances are unstable.