Method for evaluating microalgae biodiesel producing capacity
A technology of microalgae and capacity, which is applied in the field of evaluating oil production capacity of microalgae, and can solve problems such as unclear evaluation of oil production capacity, expensive equipment, and difficulty in horizontal comparison
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Embodiment 1
[0048] A method for evaluating oil-producing ability of microalgae, comprising the steps of:
[0049] (1) Chlorella sorokiniana cell collection and quenching:
[0050] Chlorella sorokiniana was cultured at different inoculation densities, and the initial inoculation density was set to 1×104 , 1×10 5 , 1×10 6 and 1×10 7 cells mL -1 , when the cells were cultured until the OD560 was 1.5, 5 samples of algae liquid were taken out for each inoculated density of microalgae, 100 mL for each, centrifuged at 3000 rpm at 4°C for 3 minutes, and the cells in the lower layer were collected, and stored at -40°C with 4 mL of metabolic stop solution. Quenching at high temperature for 5 minutes to terminate the metabolic reaction, and freeze-drying at -80°C for 4 hours to obtain freeze-dried cells;
[0051] The metabolic termination solution contains 1500 mg·L -1 NaNO 3 , 36mg·L -1 CaCl 2 2H 2 O, 75mg·L -1 MgSO 4 ·7H 2 O and 40mg·L -1 K 2 HPO 4 ·3H 2 O in methanol aqueous solut...
Embodiment 2
[0084] A method for evaluating oil-producing ability of microalgae, comprising the steps of:
[0085] (1) Chlorella sorokiniana cell collection and quenching:
[0086] Chlorella sorokiniana was cultured at different inoculation densities, and the initial inoculation density was set to 1×10 4 , 1×10 5 , 1×10 6 and 1×10 7 cells mL -1 , when the cells were cultured until the OD560 was 1.0, 4 samples were taken out of the algae liquid cultured at each inoculation density, 120 mL each, centrifuged at 5000 rpm at 4°C for 3 minutes, and the cells in the lower layer were collected, and the cells in the lower layer were collected with 3 mL of metabolic stop solution at -40°C Quench for 7 minutes to terminate the metabolic reaction, and freeze-dry at -80°C for 6 hours to obtain freeze-dried cells;
[0087] Metabolism termination liquid is the same as embodiment 1;
[0088] (2) Extract intracellular fatty acids:
[0089] Take 4 portions of freeze-dried cells obtained in step (1), ...
Embodiment 3
[0107] A method for evaluating oil-producing ability of microalgae, comprising the steps of:
[0108] (1) Chlorella sorokiniana cell collection and quenching:
[0109] Chlorella sorokiniana was cultured at different inoculation densities, and the initial inoculation density was set to 1×10 4 , 1×10 5 , 1×10 6 and 1×10 7 cells mL -1 , when the cells were cultured until the OD560 was 0.5, 3 samples were taken out of the algae liquid cultured at each inoculation density, each 150mL, centrifuged at 3000rpm at 4°C for 4min, the cells in the lower layer were collected, and the cells in the lower layer were collected and stored at -40°C with 5mL of metabolic stop solution Quenching at high temperature for 10 minutes to terminate the metabolic reaction, and freeze-drying at -80°C for 6 hours to obtain freeze-dried cells;
[0110] Metabolism termination liquid is the same as embodiment 1;
[0111] (2) Extract intracellular fatty acids:
[0112] Take 3 portions of freeze-dried ce...
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