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Nondestructive testing method for pigment content of color wheat grain

A technology of colored wheat and pigment content, applied in the field of biology and physiology, can solve problems such as research, damage to seeds, and inability to obtain grains, and achieve the effect of less use, integrity assurance, and fewer instruments

Active Publication Date: 2012-06-20
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, it is necessary to destroy the seeds to extract the pigment by this method, so that the grains that have been tested for pigment content cannot be used for further research.

Method used

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  • Nondestructive testing method for pigment content of color wheat grain
  • Nondestructive testing method for pigment content of color wheat grain
  • Nondestructive testing method for pigment content of color wheat grain

Examples

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Comparison scheme
Effect test

Embodiment 1

[0043] The color wheat sample selected in this example is the F2 generation grain color segregation population produced by crossing red-purple grain wheat and common grain-color wheat. This helps to verify the scope of application of the invention.

[0044] ． Image Acquisition of Red and Purple Wheat

[0045] Select 155 red-purple wheat grains and number the grains respectively, and place them evenly on the scanner to obtain scanned images. When measuring the gray value of colored wheat grains, the external conditions have a great influence on the gray value. This measurement is carried out under the condition that the external light source and other conditions are basically the same, so as to eliminate the error of gray value measurement caused by the different external environment. When placing colored wheat grains on the scanner, the grains should be placed neatly with approximately the same distance between the grains; the ventral groove of the grains is downward. The ...

Embodiment 2

[0106] Example 2. Utilize the method of the present invention to measure the gray value of another kind of red-purple wheat sample, put the data into a mathematical model, and calculate the pigment content of each grain:

[0107] According to the method described in step A1 of the content of the present invention, each capsule is determined successively. The gray value of red-purple wheat, the mathematical model of the gray value Xg and pigment content Eg of red-purple wheat in colored wheat is:

[0108] Eg=0.2072-0.0015Xg where: Eg—the pigment content of the grain

[0109] Xg—the gray value of the grain (0﹤Xg﹤138.13)

[0110] E.g:

[0111] The gray value of No. 1 grain Xg = 38.08 Eg=0.2072-0.0015×38.08 = 0.1501

[0112] The gray value of No. 2 grain Xg = 44.04 Eg=0.2072-0.0015×44.04 = 0.1411

[0113] The gray value of No. 3 grain Xg = 51.19 Eg=0.2072-0.0015×51.19 = 0.1304

[0114] The gray value of No. 4 grain Xg = 60.17 Eg=0.207...

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Abstract

The invention relates to a nondestructive testing method for the pigment content of a color wheat grain. In the nondestructive testing method, a computer is used to determine the gray value of color wheat on the premise of not destroying the grain, thus the pigment content of the color wheat grain can be directly acquired, and a mathematical model of the gray value and the pigment content of the color wheat grain can be simultaneously constructed; the gray value of the wheat grain can be used for directly reflecting the depth of the color of the wheat grain; and the depth of the color wheat grain is related to the pigment content of the color wheat grain, so that the pigment content of the wheat grain can be reflected by determining the gray value of the color wheat grain, thus the integrity of the color wheat grain is ensured, so as to carry out next study and application on the color wheat. The mathematical model which is provided by the invention and can be used for showing the pigment content of the color wheat grain by utilizing the gray value can be used for conveniently and rapidly calculating the pigment content of the color wheat grain.

Description

technical field [0001] The invention relates to a non-destructive detection method for the pigment content of colored wheat grains, which belongs to the physiological discipline in the field of biology. Background technique [0002] Colored wheat refers to wheat whose seed coat or aleurone layer color is different from that of ordinary grain-colored wheat. Compared with ordinary wheat, the most intuitive difference between colored wheat and common wheat is that the grain color is darker. The color wheats that have been reported so far include purple kernels, black kernels, blue kernels, blue-purple kernels, and green kernels. Colored wheat is created by breeders after years of careful selection by using breeding methods such as "chemical mutagenesis", "physical mutagenesis", "distant hybridization" and "gene introduction". Foreign countries have reported sterile hexaploid triticale in 1913, and reported fertile hexaploid triticale in 1938. In China, it was reported in 198...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/33
Inventor 尹燕枰黄玮王振林唐晓珍孙兰珍
Owner SHANDONG AGRICULTURAL UNIVERSITY
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