Method for forecasting leading chromatographic peak shape under multi-order programmed temperature condition
A temperature-programmed, chromatographic peak technology, applied in the field of gas chromatography, can solve the problems of chromatographic peak shape prediction that have not been reported yet
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0082] Instruments: HP6890 gas chromatograph, hydrogen flame ionization detector, 6890 gas chromatograph workstation;
[0083] Chromatographic column: non-polar HP-5 (5% phenylmethylpolysiloxane) column;
[0084] Conditions: The temperature of the detector is 250°C, and the temperature of the injection port is 250°C;
[0085] Carrier gas: use high-purity nitrogen gas (purity not less than 99.999%), constant flow operation mode, that is, the carrier gas is at the outlet of the column,
[0086] The mass flow rate is kept constant at 1mL / min;
[0087] Injection method: split injection, the split ratio is 50:1, each injection volume is 0.2 μL, and the concentration is 1 μg / mL;
[0088] (1) Select heptanoic acid as the compound to be tested, and measure its retention time at six constant temperatures of 30°C, 50°C, 100°C, 150°C, 200°C and 250°C on the HP-5 column, which are 25.01min, 14.32min, 7.30min, 3.07min, 2.34min and 2.08min;
[0089] (2) Take the virtual dead time ...
Embodiment 2
[0106] Experimental apparatus, chromatographic column, condition, carrier gas and sample injection mode are the same as embodiment 1;
[0107] (1) Heptanoic acid was selected as the compound to be tested, and its retention times at six constant temperatures of 30°C, 50°C, 100°C, 150°C, 200°C and 250°C were measured on the HP-5 column, which were 25.01min, 14.32min, 7.30min, 3.07min, 2.34min and 2.08min;
[0108] (2) Take the virtual dead time τ=1.85min, and calculate the retention factors of heptanoic acid at six constant temperatures according to formula 1, which are respectively: 2.53, 1.91, 1.08, -0.42, -1.33 and -2.12. At lower temperatures, the distribution of heptanoic acid between the two phases is relatively slow, and is affected by mass transfer resistance diffusion items, etc. Therefore, when using Equation 2 to fit the curves of the relationship between retention factor and temperature at six constant temperatures , the above parameters need to be corrected, and fi...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com