Method for preparing boron-doped diamond film electrode for detecting dopamine

Abstract

The invention discloses a method for preparing a boron-doped diamond (BDD) film electrode for detecting dopamine (DA), characterized by using a sharp tip-shaped tungsten filament as a substrate, depositing a BDD film on the tungsten filament, activating the surface amino groups of the diamond film, then directly preparing a tyrosinase modification layer on the BDD film, wherein the tungsten filament is connected with a guide wire through a silver paste. The BDD film electrode is used as a biological probe, cyclic voltammetry is used to detect the DA content, and the concentration of DA in vivo can be detected in real time. According to the invention, the electrode prepared by the invention has good repeatability and electrochemical stability for detecting DA, has high sensitivity and strong selectivity, the modified BDD electrode can effectively separate oxidation peaks of DA and ascorbic acid (AA) in a solution containing AA that is many times DA and an ideal linear response relation is obtained in a wide range; the prepared electrode has extremely good anti pollution capability and long life, can not generate passivation pollution, and the needle-type electrode is beneficial for implanting operation of sensors in surgery.

Description

technical field [0001] The invention relates to the field of diamond film electrodes and biosensors, in particular to a method for preparing a boron-doped diamond film electrode for detecting dopamine. Background technique [0002] Dopamine (DA for short), as an important catecholamine neurotransmitter in the hypothalamus and pituitary gland, plays an important role in the control and prevention of health and disease. Changes in its content can lead to some diseases such as schizophrenia and Parkinson's disease. In addition, dopamine has the function of exciting the heart and increasing renal blood flow, and can be used to treat hemorrhagic, cardiogenic and septic shock. Therefore, the research on its determination method is of great significance in clinical application and diagnosis of related diseases. [0003] The traditional methods for detecting dopamine mainly include microdialysis, gas chromatography-mass spectrometry, spectrophotometry, ion chromatography, chemilum...

AI Technical Summary

Problems solved by technology

However, so far, there are few studies on the utilization of BDD electrodes as substrate materials for amperometric biosensors

[0009] In addition, at present, the artificial synthesis of high-purity, nano-scale boron-doped diamond films mostly uses the MPCVD method, and most of them use MPCVD to deposit films on two-dimensional planar substrates, which limits the shape of film electrodes to a certain extent. Therefore, traditional boron-doped diamond films The electrode used for detection is limited by its shape and size, which is not conducive to the flexibility and sensitivity of detection

The main performance is that it is not conducive to implanting electrodes in the brain, thus limiting the clinical application of electrodes to detect dopamine

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