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Method rapidly identifying cucumber breeding material with heat resistance and moisture resistance without damaging seedling stage

A non-destructive, moisture-resistant technology, applied in horticultural methods, botanical equipment and methods, gardening tools/equipment, etc., can solve the problems of high cost, complicated technology, shortening the breeding cycle, etc., and achieve high accuracy and relevance Good, easy to learn and operate effect

Inactive Publication Date: 2013-07-31
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Non-destructive rapid identification of cucumber heat resistance and humidity resistance at the seedling stage can improve breeding efficiency and shorten the breeding cycle. The previous breeding methods required a lot of physiological and biochemical analysis, which was costly and complex.

Method used

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  • Method rapidly identifying cucumber breeding material with heat resistance and moisture resistance without damaging seedling stage
  • Method rapidly identifying cucumber breeding material with heat resistance and moisture resistance without damaging seedling stage
  • Method rapidly identifying cucumber breeding material with heat resistance and moisture resistance without damaging seedling stage

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Embodiment 1

[0014] 2 copies of North China open field cucumbers, 2 copies of North China spring and autumn greenhouse cucumbers, 2 copies of North China and European hybrid cucumbers, 2 copies of Thai native cucumbers, Thai native and North China hybrid cucumbers F 1 2 copies, Thai native type and North China type hybrid cucumber F 2 2 servings, 1 European cucumber, 1 Japanese cucumber.

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Abstract

The invention discloses a method rapidly identifying a cucumber breeding material with heat resistance and moisture resistance without damaging a seedling stage, which comprises the following steps carried out in a manual simulation environment with daytime / night=12hr / 12hr, daytime temperature of 35 DEG C, night temperature of 25 DEG C, daytime humidity of 80%, night humidity of 100% and daytime illumination intensity of 40,000lux: (1) carrying out first screening on the seventh day after cucumber sowing, and selecting seedlings with the length of hypocotyls not exceeding 4cm; (2) on the ninth day after sowing, selecting the seedlings where first main leaves have the sizes not exceeding 4*5cm and the main leaves and edges of cotyledons are free of thermal burn; and (3) respectively inoculating plasmopara viticola and zucchini yellow mosaic virus (ZYMV) on the seedlings selected in the step (2) at the period of a third main leaf. The seedlings having heat resistance and moisture resistance to the plasmopara viticola and the ZYMV are the cucumber breeding material with the heat resistance and the moisture resistance. The method rapidly identifying the cucumber breeding material with the heat resistance and the moisture resistance without damaging the seedling stage causes no damage to the seedlings, can identify the cucumber breeding material with the heat resistance and the moisture resistance through three simple morphological characteristic indexes and is learned and operated easily. Besides, the method is good in relevance and high in accuracy rate.

Description

Technical field [0001] The invention relates to a method for rapid identification of cucumber breeding materials with heat resistance and moisture resistance without damage at the seedling stage. Background technique [0002] With global warming, the average temperature in North China gradually rises, summers are prolonged, winters are shortened, rainwater distribution is unbalanced, and the proportion of cucumbers in the protected area is increasing. The heat resistance and humidity resistance of cucumbers will become important constraints on the development of cucumber production in the future. Factors. Taking precautions, strengthening the research on heat resistance and humidity resistance of cucumbers, and screening out a batch of related resources is an important topic facing cucumber breeders. The non-destructive and rapid identification of cucumber heat resistance and moisture tolerance at the seedling stage can improve the breeding efficiency and shorten the breeding cyc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/00A01G7/06
Inventor 张峰刘立功
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES