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Method for extracting scallop polysaccharide by utilizing scallop waste liquid

A technology for waste liquid and shellfish polysaccharide, which is applied in the field of polysaccharide preparation, can solve problems such as environmental pollution and resource waste, and achieve the effects of good water solubility, strong operability, and advanced and reasonable preparation technology.

Inactive Publication Date: 2012-07-04
SHANDONG HOMEY AQUATIC DEV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the disadvantages in the prior art that untreated scallop waste liquid is likely to cause environmental pollution and waste of resources, the present invention provides a method for extracting scallop polysaccharides from scallop waste liquid with reasonable production process and strong operability

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A method for extracting scallop polysaccharides from scallop waste liquid, characterized in that: through the following processes

[0022] a. Pretreatment of scallop waste liquid Because scallop waste liquid is rich in nutrients and high in organic matter content, it is suitable for the growth and reproduction of bacteria. If the indoor temperature is high, the waste liquid will rot within a few hours. Therefore, weigh the scallop waste liquid, first use 2mol / L dilute hydrochloric acid to adjust the pH value to 4.0, heat to 80°C, carry out sterilization treatment, then let it stand for 2 hours, and then filter it with a 100-mesh filter cloth to remove the scallop waste liquid. protein and impurities, to obtain the filter cloth filtrate;

[0023] b. Membrane filtration Reduce the temperature of the filter cloth filtrate obtained in step a to 40°C, first filter through a 100KDa polyethersulfone membrane filtration device, and control the working pressure at 0.3Mpa; the fi...

Embodiment 2

[0030] A method for extracting scallop polysaccharides from scallop waste liquid, through the following process

[0031] a. Pretreatment of scallop waste liquid Weigh the scallop waste liquid, use 1mol / L dilute hydrochloric acid to adjust the pH value to 4.5, heat to 90°C, carry out sterilization treatment, then let it stand for 2.5 hours, and then filter it with a 120-mesh filter cloth , remove the protein and impurities in the scallop waste liquid to obtain the filter cloth filtrate;

[0032] b. Membrane filtration Reduce the temperature of the filter cloth filtrate obtained in step a to 45°C, first filter through a 100KDa polyethersulfone membrane filtration device, and control the working pressure at 0.4Mpa; the filtrate then passes through a 50KDa polyethersulfone membrane Filtration equipment is used to filter, and the working pressure is also controlled at 0.4Mpa to obtain 50KDa initial retentate;

[0033] When the volume of the 50KDa initial retentate reaches twice th...

Embodiment 3

[0041] A method for extracting scallop polysaccharides from scallop waste liquid, through the following process

[0042] a. Pretreatment of scallop waste liquid Weigh the scallop waste liquid, adjust the pH value to 5.0 with 1.5mol / L dilute hydrochloric acid, heat to 100°C, perform sterilization treatment, then let it stand for 3 hours, and then filter it with a 120-mesh filter cloth , remove the protein and impurities in the scallop waste liquid to obtain the filter cloth filtrate;

[0043] b. Membrane filtration Reduce the temperature of the filter cloth filtrate obtained in step a to 50°C, first filter through a 100KDa polyethersulfone membrane filtration device, and control the working pressure at 0.5Mpa; the filtrate then passes through a 50KDa polyethersulfone membrane Filtration equipment is used to filter, and the working pressure is also controlled at 0.5Mpa to obtain 50KDa initial retentate;

[0044] When the volume of the obtained 50KDa initial retentate reaches tw...

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Abstract

The invention relates to a method for extracting scallop polysaccharide by utilizing scallop waste liquid, which includes adjusting pH value of the scallop waste liquid, conducting heating sterilization processing, conducting coarse filtering to remove proteins and impurities, then conducting film filtering to obtain retaining liquid of 100KDa-50KDa, conducting alcohol precipitation on the retaining liquid to obtain scallop polysaccharide precipitate, and conducting alcohol washing, freezing and drying to obtain the scallop polysaccharide. Polysaccharide prepared by the method is high in yield and purity. The preparation method is advanced and reasonable in process, strong in operational performance and suitable for industrial production.

Description

technical field [0001] The invention relates to the preparation of polysaccharides, in particular to a method for extracting scallop polysaccharides from scallop waste liquid. Background technique [0002] Scallop is a general term for the mollusc phylum Scallopidae Chlamys farreri, Gulf scallop and Ezo scallop, and is one of the important economic seafood in my country. Because of the high protein content in scallops, and rich in various amino acids, highly unsaturated fatty acids and trace elements, they are very popular among the public. [0003] Since the 1980s, my country's aquaculture industry has developed rapidly, and the raft culture of scallops has been widely popularized. With the large-scale processing of scallops, the waste liquid generated during the processing is also gradually increasing. If the waste liquid is not treated, it will cause a great waste of resources and environmental pollution. According to measurements: every kilogram of scallop processing ...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 孟秀梅刘昌衡胡炜袁文鹏贾爱荣张绵松孙永军刘新张永刚夏雪奎
Owner SHANDONG HOMEY AQUATIC DEV
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