Method for extracting scallop polysaccharide by utilizing scallop waste liquid
A technology for waste liquid and shellfish polysaccharide, which is applied in the field of polysaccharide preparation, can solve problems such as environmental pollution and resource waste, and achieve the effects of good water solubility, strong operability, and advanced and reasonable preparation technology.
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[0020] Example 1
[0021] A method for extracting scallop polysaccharides from scallop waste liquid, characterized in that: through the following processes
[0022] a. Pretreatment of scallop waste liquid Because scallop waste liquid is rich in nutrients and high in organic matter content, it is suitable for the growth and reproduction of bacteria. If the indoor temperature is high, the waste liquid will rot within a few hours. Therefore, weigh the scallop waste liquid, first use 2mol / L dilute hydrochloric acid to adjust the pH value to 4.0, heat to 80°C, carry out sterilization treatment, then let it stand for 2 hours, and then filter it with a 100-mesh filter cloth to remove the scallop waste liquid. protein and impurities, to obtain the filter cloth filtrate;
[0023] b. Membrane filtration Reduce the temperature of the filter cloth filtrate obtained in step a to 40°C, first filter through a 100KDa polyethersulfone membrane filtration device, and control the working pressu...
Example Embodiment
[0029] Example 2
[0030] A method for extracting scallop polysaccharides from scallop waste liquid, through the following process
[0031] a. Pretreatment of scallop waste liquid Weigh the scallop waste liquid, use 1mol / L dilute hydrochloric acid to adjust the pH value to 4.5, heat to 90°C, carry out sterilization treatment, then let it stand for 2.5 hours, and then filter it with a 120-mesh filter cloth , remove the protein and impurities in the scallop waste liquid to obtain the filter cloth filtrate;
[0032] b. Membrane filtration Reduce the temperature of the filter cloth filtrate obtained in step a to 45°C, first filter through a 100KDa polyethersulfone membrane filtration device, and control the working pressure at 0.4Mpa; the filtrate then passes through a 50KDa polyethersulfone membrane Filtration equipment is used to filter, and the working pressure is also controlled at 0.4Mpa to obtain 50KDa initial retentate;
[0033] When the volume of the 50KDa initial retent...
Example Embodiment
[0040] Example 3
[0041] A method for extracting scallop polysaccharides from scallop waste liquid, through the following process
[0042] a. Pretreatment of scallop waste liquid Weigh the scallop waste liquid, adjust the pH value to 5.0 with 1.5mol / L dilute hydrochloric acid, heat to 100°C, perform sterilization treatment, then let it stand for 3 hours, and then filter it with a 120-mesh filter cloth , remove the protein and impurities in the scallop waste liquid to obtain the filter cloth filtrate;
[0043] b. Membrane filtration Reduce the temperature of the filter cloth filtrate obtained in step a to 50°C, first filter through a 100KDa polyethersulfone membrane filtration device, and control the working pressure at 0.5Mpa; the filtrate then passes through a 50KDa polyethersulfone membrane Filtration equipment is used to filter, and the working pressure is also controlled at 0.5Mpa to obtain 50KDa initial retentate;
[0044] When the volume of the obtained 50KDa initial ...
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