Determining method for total flavone content of blumea balsamifera

Abstract

The invention discloses a determining method for the total flavone content of blumea balsamifera or blumea balsamifera extractive or materials having the same total flavone with the blumea balsamifera, relating to an ultraviolet-visible spectrophotometry. The determining method comprises the following steps of: preparing a gradient standard solution by using rutin or blumeatin (3, 3', 5, 7-tetramethyl-4'-methoxy flavanone) reference material, preparing a solution to be tested by using a blumea balsamifera medical material or the blumea balsamifera extractive, directly determining the absorbance of the gradient standard solution under the wavelength of 271nm, and establishing a standard curve; and determining the absorbance of the solution to be tested again, finding out the concentration of the solution to be tested from the standard curve, and finally working out the total flavone content of the blumea balsamifera medical material or extractive according to the dilution multiple of solution preparation. According to the determining method, an isoabsorptive point is adopted for absorbing wavelengths to determine the total contents of the two types of materials with different ultraviolet absorptions; and the determining method has the advantages of strong pertinence, accurate and reliable results, good accuracy, stability and repeatability, high degree of accuracy, easiness in control, simpleness and convenience in operation and can be used for the quality control of the blumea balsamifera medical material or extractive.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine analysis, and relates to a quality control method for traditional Chinese medicinal materials or extracts, in particular to a method for measuring the content of total flavonoids containing two types of flavonoids, and in particular to a method for determining the extracts of aina incense or aina incense. The method for the content of total flavonoids in a substance or a substance with the same total flavonoid composition of Ainaxiang. Background technique [0002] Ainaxiang is the dry aerial part of Ainaxiang Blumea balsamifera (L.) DC. Chinese medicine is also called Dafengai. Mainly produced in Guangxi and Guizhou. According to "Guangxi Traditional Chinese Medicine Standards" (1990 edition), Ainaxiang (Dafeng Ai) is "pungent, bitter, and warm", and has the effects of "warming and promoting blood circulation, regulating menstruation, dispelling wind and removing dampness". Modern p...

AI Technical Summary

Problems solved by technology

However, the method for measuring total flavonoids after color development is relatively cumbersome, and only the color reaction needs 30 minutes; the stability is also relatively poor, as in the two documents of the above-mentioned determination of total flavonoids of Ainaxiang and Diangui Ainaxiang. The maximum absorption wavelength Inconsistent, one is 500nm, the other is 505nm

The method of direct determination of total flavonoids without color development is relatively simple. For medicinal materials or extracts containing a single type of flavonoids or mainly a single type of flavonoids, this determination method has a small error; however, for both The medicinal materials or extracts of two types of flavonoids (such as Ainaxiang), because the maximum absorption wavelength of flavonoids is 254nm, it happens to be the trough of the ultraviolet absorption of dihydroflavones, and the maximum absorption wavelength of dihydroflavones is 289nm, which happens to be the trough of flavones ultraviolet absorption. The trough of absorption (as attached figure 1 ), at this time, the result obtained by directly measuring the total flavonoids using a flavonoid maximum absorption wavelength will be less than the actual content of the sample, and the measurement results will have a large error due to the interference of another flavonoid

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