Chinese cabbage TT8 gene family and application thereof

A gene family and gene technology, applied in the field of genetic engineering, achieves good results in application prospects

Inactive Publication Date: 2012-07-11
SOUTHWEST UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are no reports on the number of members, protein characteristics, evolutionary relationship, tissue specificity of expression, relationship betw

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  • Chinese cabbage TT8 gene family and application thereof
  • Chinese cabbage TT8 gene family and application thereof
  • Chinese cabbage TT8 gene family and application thereof

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Embodiment Construction

[0040] Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. The experimental method that does not indicate specific conditions in the preferred embodiment is usually according to conventional conditions, such as described in the Molecular Cloning Experiment Guide (Third Edition, J. Sambrook et al., translated by Huang Peitang, etc., Science Press, 2002) conditions, or as recommended by the manufacturer.

[0041] The plant materials used in the preferred embodiment: the cabbage materials are all from the Brassica napus subspecies (B.rapa ssp.oleifera), including the typical black seed line 06K130 and the yellow seed line 06K124; the cabbage materials are all from the kale variety (B. oleracea var.acephala), including typical black seed line 06K158 and yellow seed line 06K165; Brassica napus materials include typical black seed line 5B, black seed variety Xiangyou 15, near-isogenic black seed line L1...

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Abstract

The invention discloses a Chinese cabbage TT8 gene family comprising two members, namely a BrTT8-1 gene with a full-length cDNA sequence shown as SEQ ID No.2 and a BrTT8-2 gene with a full-length cDNA sequence shown as SEQ ID No.4. The gene family can be applied to the molecular breeding based on seed traits such as seed coat color, seed size and the like of a brassica plant.

Description

[0001] This application is a divisional application with the application number 201010281920.7, the filing date is 2010-09-15, and the invention name is "Brassica napus and its parent species Chinese cabbage and Brassica TT8 gene family and its application". technical field [0002] The invention relates to the technical field of genetic engineering, in particular to Brassica napus and its parent species Chinese cabbage (Brassica rapa) and cabbage (Brassica oleracea) TT8 (TRANSPARENT TESTA 8, transparent seed coat 8; also known as bHLH42, BASIC HELIX - LOOP-HELIX 42, basic helix-loop-helix 42) gene family and applications thereof. Background technique [0003] The genus Brassica (Brassica) of the Brassicaceae (Brassicaceae) includes many oil crops, vegetables and ornamental plant species. It provides humans with nutritious edible oils, vegetables and ornamental plants, and provides feed for animal husbandry. It has an important economic value. Among the Brassica species, th...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/63C12N15/82C12N1/15C12N1/19C12N1/21A01H5/00
Inventor 柴友荣殷家明赵文军谢伶俐肖霞李加纳林呐张瑞熙许本波
Owner SOUTHWEST UNIVERSITY
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