Biological nanometer material sensor electrode and manufacturing method and using method thereof

A technology of biological nanomaterials and sensor electrodes, which is applied in the analysis of materials, material analysis by electromagnetic means, instruments, etc., can solve the problems of limited practical value, low sensitivity, and narrow linear response range, and achieves short response time and sensitivity. The effect of high, linear response range

Inactive Publication Date: 2012-07-11
石瑾 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of existing electrochemical sensors are low sensitivity a

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A method for manufacturing a bionanomaterial sensor electrode-glucose sensor electrode in this embodiment comprises the following steps:

[0035] Unless otherwise specified, all solutions were prepared with deionized water (resistivity 18.2 MΩ*cm).

[0036] 1) Carbon nanotube immobilization

[0037] The carbon nanotubes are dissolved in a Nafion solution with a concentration of 0.5%. The Nafion solution is a copolymer of polytetrafluoroethylene and perfluoro-3,6-diepoxy-4-methyl-7-decene-sulfuric acid. Contain 0.5 mg of carbon nanotubes in a milliliter of Nafion solution, ultrasonically disperse the mixture for 1 hour to obtain a black uniform dispersion, take the dispersion and evenly cover the top surface of the platinum / iridium alloy electrode, and let it stand at room temperature until it is dry; the electrode is divided into The top (the part to be modified) and the tail, the electrodes are connected to various devices through the tail, and the top is the probe p...

Embodiment 2

[0044] A method for making a biological nanomaterial sensor-a lactic acid sensor electrode, comprising the following steps:

[0045] 1. Carbon nanotube immobilization

[0046]Carbon nanotubes are dissolved in a Nafion solution with a concentration of 5%. The Nafion solution is a copolymer of polytetrafluoroethylene and perfluoro-3,6-diepoxy-4-methyl-7-decene-sulfuric acid. Contain 5 milligrams of carbon nanotubes in the milliliter Nafion solution, ultrasonically disperse the mixture for 2 hours to obtain a uniform black dispersion, take the dispersion and evenly cover the top surface of the electrode obtained by platinum black electroplating, and let it stand at room temperature until dry; For the top (the part to be modified) and the tail, the electrodes are connected to various devices through the tail, and the top is the probe part;

[0047] The electrode production method obtained by platinum black electroplating is as follows:

[0048] Connect the tail end of the platin...

Embodiment 3

[0056] A kind of bionano material sensor electrode of the present embodiment-the preparation method of alcohol sensor electrode comprises the following steps:

[0057] 1) Carbon nanotube immobilization

[0058] Carbon nanotubes are dissolved in a Nafion solution with a concentration of 10%. The Nafion solution is a copolymer of polytetrafluoroethylene and perfluoro-3,6-diepoxy-4-methyl-7-decene-sulfuric acid. Contain 10 mg of carbon nanotubes in a milliliter of Nafion solution, ultrasonically disperse the mixture for 3 hours to obtain a black uniform dispersion, take the dispersion and evenly cover the top surface of the platinum / iridium alloy electrode, and let it stand at room temperature until it is dry; the electrode is divided into The top (the part to be modified) and the tail, the electrodes are connected to various devices through the tail, and the top is the probe part;

[0059] 2), enzyme immobilization

[0060] Dissolve alcohol oxidase in phosphate buffer to prepa...

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Abstract

The invention relates to a biological nanometer material sensor electrode and a manufacturing method and a using method of the biological nanometer material sensor electrode, belonging to the technical field of a biological nanometer material sensor. The manufacturing method comprises the following steps: (1) fixing a carbon nano-tube; and (2) immobilizing enzyme. The carbon nano-tube and a platinum black nano material adopted in the method have a function of catalytic electrochemical reaction, and the carbon nano-tube and the platinum black nano material are fixed on the surface of the sensor, so that the response time, sensitivity, linear response range and anti-jamming capability of the sensor can be significantly improved, and furthermore, the application range of the sensor is greatly increased. Compared with the current glucometer, the sensor electrode has the advantages of real-time detection, 0.8 seconds of response time, high sensitivity, large linear response range (10 muM-22muM glucose), strong anti-jamming capability, recycling, low production cost and the like, and the 10 muM glucose (as the minimum level ) can be detected.

Description

technical field [0001] The invention relates to a bionano material sensor electrode, a manufacturing method and a use method thereof, and belongs to the technical field of bionano material sensors. Background technique [0002] Existing electrochemical sensors use enzymes as biorecognition molecules. When used, the enzyme will specifically bind the analyte molecule, convert the analyte molecule into an intermediate molecule that can be oxidized, and then under the action of the working voltage, the intermediate molecule is oxidized , generate a current, and measure the concentration of the molecule to be measured by measuring the current. The disadvantages of existing electrochemical sensors are low sensitivity and narrow linear response range, so the practical value is extremely limited. Contents of the invention [0003] The purpose of the present invention is to solve the shortcomings of the above-mentioned prior art, and provide a bionano-material with real-time detec...

Claims

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Application Information

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IPC IPC(8): G01N27/327
Inventor 石谨戴维马歇尔・波特菲尔德埃里克・斯科特・麦克勒莫
Owner 石瑾
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