Microfluidic chip for isoelectric focusing separation

A microfluidic chip and isoelectric focusing technology, which is applied in the direction of material analysis, material analysis, and DNA preparation through electromagnetic means, can solve the problems that the separation resolution needs to be further improved and the separation effect cannot be achieved, and achieve good results and Practical significance, reagent saving, simple production effect

Inactive Publication Date: 2012-07-11
XIAMEN UNIV
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Problems solved by technology

However, the reported isoelectric focusing method using microfluidic chips still mainly continues the cIEF method, and seldom improves the microchannel structure to improve the separation performance. For samples with relatively close isoelectric points, it is still unable to achieve Very good separation effect, the separation resolution needs to be further improved

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  • Microfluidic chip for isoelectric focusing separation
  • Microfluidic chip for isoelectric focusing separation
  • Microfluidic chip for isoelectric focusing separation

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Embodiment 1

[0021] see figure 1 and 2 , the shrinkage region 4 and the expansion region 5 of the microchannel both have a length of 40 μm and a width of 50 μm and 100 μm respectively, and the ratio is 2. The connecting region 6 between the constricted region 4 and the enlarged region 5 has a length of 10 μm. The length of the repeating structure composed of the three parts of channel constriction region 4, expansion region 5 and connection region 6 can be 100 μm. Channels of different widths have different resistivities in the electric field and have different voltage drops, enabling nonlinear pH gradients during isoelectric focusing. The IPG strips used in conventional isoelectric focusing have several specifications, and the lengths are 7cm, 11cm, 17cm, 18cm and 24cm. If microchannels with the same length as IPG strips are made, 700, 1100, 1700, 1800 and 2400 repeating structures can be processed respectively. If each repeat structure accommodates one sample, peak capacities of 700,...

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Abstract

The invention relates to a microfluidic chip, particularly to a microfluidic chip for isoelectric focusing separation, which is provided with two substrates that are bonded in reversible or non-reversible way, wherein a micro-channel and an access hole are machined in one substrate; the access hole serves a liquid storage tank; the micro-channel is provided with an extracting area and an expanding area; a transitional connecting area is arranged between the extracting area and the expanding area; and the connecting area is provided with an inclination angle. Under the premise that the pH gradient is a set value, the cyclical variation of the width of the micro-channel is utilized to enable the electric field distribution in the micro-channel to regularly vary, so as to form the non-linear pH gradient, as a result, the protein samples with similar isoelectric points can be separated in higher resolution. The microfluidic chip has the characteristics that the microfluidic chip has small size, saves reagent, is quick to separate, is convenient for integration and automation, can machine thousands of repeated micro-structures once for all, has low cost, is simple and convenient to make and operate, has high efficiency, and has excellent effect and practical significance in the isoelectric focusing separation.

Description

technical field [0001] The invention relates to a microfluidic chip, in particular to a microfluidic chip for isoelectric focusing and separation which utilizes a zigzag microchannel to realize high-resolution isoelectric focusing and separation. Background technique [0002] Isoelectric focusing (IEF) is the use of ampholyte carriers to form a continuous pH gradient in an electric field. Biomolecules such as proteins have a specific isoelectric point and will migrate to a pH consistent with the isoelectric point in the pH gradient. A method to achieve the purpose of separation, determination and identification. [0003] The early isoelectric focusing electrophoresis adopts vertical tube type, and in recent years, ultra-thin layer horizontal plate type isoelectric focusing electrophoresis has been developed, and polyacrylamide gel is generally used as the support medium for anti-convection and diffusion (D.J. Buhler, E. Pei Tess, R. Coulter, R. Pentman Chinese patent CN1014...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/447C07K1/28C12N15/10
Inventor 陈宏付广春戴思敏张博
Owner XIAMEN UNIV
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