Probiotic derived non-viable material for allergy prevention and treatment

A composition, mixture technology, applied in the field of diet or nutritional products

Inactive Publication Date: 2012-07-11
MJN U S HLDG LLC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is currently not possible to use only the culture supernatant itself as the basis for non-viable probiotic materials especially for infant formula etc.

Method used

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  • Probiotic derived non-viable material for allergy prevention and treatment
  • Probiotic derived non-viable material for allergy prevention and treatment
  • Probiotic derived non-viable material for allergy prevention and treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0080] In batch fermentation, LGG is grown under physiological conditions. The pH was kept constant at pH 6 by adding 33% NaOH and the temperature was maintained at 37°C. Stirrer speed is 50 rpm, headspace with N 2 rinse. The following medium (modified MRS broth) was provided (Table 1).

[0081] Table 1

[0082]

[0083]

[0084] Bacterial growth is depicted in Figure 1(a) and (b).

[0085] Figure 1a The evolution of pH, amount of titrated NaOH (33%) (DM base = dose monitoring base) and OD600 during the LGG fermentation is shown. Refer to the legend given in the figure. pH and OD600 measurements allow the determination of bacterial growth in the fermenter; where the need to add NaOH to maintain a pH of 6 correlates with lactate production (i.e. a measure of bacterial metabolic activity), OD600 correlates with the number of bacteria in the fermenter Density measurement.

[0086] exist Figure 1b The middle vertical axis represents, on a logarithmic scale, the bac...

Embodiment 2

[0089] Similar to Example 1, LGG culture was carried out on the basis of a modified medium. In the absence of Tween, the oil was added as follows:

[0090] (a) Oleic acid in concentrations of 1 g / kg, 2 g / kg and 4 g / kg.

[0091] (b) Linseed oil In concentrations of 1 g / kg, 2 g / kg and 4 g / kg.

[0092] (c) Olive oil In concentrations of 1 g / kg, 2 g / kg and 4 g / kg.

[0093] (d) Rapeseed oil In concentrations of 1 g / kg, 2 g / kg and 4 g / kg.

[0094] (e) Sunflower oil In concentrations of 1 g / kg, 2 g / kg and 4 g / kg.

[0095] In all of these tests (a)-(e), successful LGG growth was observed, comparable to growth in Tween-containing media. Other than that, it is amazing to be able to grow LGG successfully also without adding Tween or any oil.

Embodiment 3

[0097] In this example, the supernatant obtained as in Example 1 was screened for anti-allergic and anti-inflammatory activities using RAW 264 cells (mouse macrophage cell line) in an in vitro model recognized in the art. RAW cell cultures showed substantially increased production of the regulatory cytokine IL-10 during incubation with MJ2 supernatant sample harvests of LGG cultures compared to other supernatant sample harvests. See figure 2 .

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PUM

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Abstract

Disclosed is a process for the preparation of a non-viable probiotic composition having anti-allergic (i.e. allergy preventive and / or anti-allergic in a therapeutic sense) properties, the process comprising the steps of (a) subjecting LGG to cultivation in a suitable culture medium using a batch process; (b) harvesting the culture supernatant at a late exponential phase of the cultivation step; (c) removing liquid contents from the culture supernatant so as to obtain the composition. Also disclosed is a composition comprising a proteinaceous mixture,said composition being obtainable by the above process, and the use thereof in a dietetic product preferably for targeting expecting mother's, infants, or children as well as application in food for specific medical purposes.

Description

field of invention [0001] The present invention relates to a method for harvesting inactive bioactive material from probiotic strain Lactobacillus rhamnosus Goldin Gorbach (Lactobacillus rhamnosus Goldin Gorbach, LGG). In particular, the invention relates to a method for the preparation of an antiallergic probiotic material obtainable by said harvesting method and to a dietary or nutritional product comprising said probiotic material. Background of the invention [0002] Lactobacillus GG (Lactobacillus G.G., strain ATCC 53103) is a bacterium naturally present in the human digestive tract. It is a much-studied bacterium that is generally believed to have health benefits. It is recognized as a probiotic and is therefore incorporated into many nutritional products such as dairy products, nutritional supplements, infant formula, etc. [0003] Probiotics are currently defined in the art as live microorganisms that, when administered in adequate amounts, provide a health benefit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/38A23L1/03A61K35/74C12R1/225A23L29/00
CPCC12N1/20A23L1/3014A23L1/308A61K35/74A23L1/0345A23L1/3008C12N1/38A23L1/296A23L29/065A23L33/40A23L33/12A23L33/135A23L33/21A61P37/00A61P37/08A61K2300/00A23V2200/304C12R2001/225
Inventor E·A·F·范托尔W·M·拉塞尔U·赫尔斯H·伦斯H·加恩M·布拉克斯马M·J·范德维尔夫K·M·奥弗坎普
Owner MJN U S HLDG LLC
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