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Natural pullulan nano drug carrier and method for preparing same

A nano-drug carrier, pullulan polysaccharide technology, applied in the direction of pharmaceutical formulations, non-active ingredients medical preparations, etc., to achieve the effect of improving the therapeutic effect, good dispersion and easy access

Active Publication Date: 2012-07-25
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the particle size of drug-loaded nanoparticles reaches 803.6nm at pH=7.4, and reaches 1140nm at p

Method used

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  • Natural pullulan nano drug carrier and method for preparing same
  • Natural pullulan nano drug carrier and method for preparing same
  • Natural pullulan nano drug carrier and method for preparing same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The processing steps of the present embodiment are as follows:

[0037] (1) Add isopropanol to the natural pullulan aqueous solution, then add sodium hydroxide aqueous solution, stir at normal pressure and 50°C until a transparent solution is formed; the natural pullulan aqueous solution is deionized Prepared with water, the concentration is 0.1g / ml, the volume ratio of the isopropanol to the natural pullulan aqueous solution is 1:10; the sodium hydroxide aqueous solution is prepared with deionized water, the concentration is 0.1g / ml, and the hydroxide The amount of sodium aqueous solution is limited with the mol ratio=1:1 of the glucose unit of natural pullulan polysaccharide in the mixed solution and sodium hydroxide;

[0038](2) sodium chloroacetate aqueous solution and isopropanol are joined in the mixed solution prepared by step (1) simultaneously, under normal pressure, 50 ℃ of reaction 2 hours under stirring; The concentration of described sodium chloroacetate aq...

Embodiment 2

[0046] The processing steps of the present embodiment are as follows:

[0047] (1) Add isopropanol to the natural pullulan aqueous solution, then add sodium hydroxide aqueous solution, stir at normal pressure and 80°C until a transparent solution is formed; the natural pullulan aqueous solution is prepared with deionized water , the concentration is 0.5g / ml, the volume ratio of the isopropanol to the natural pullulan aqueous solution is 1:7 / 3; the sodium hydroxide aqueous solution is prepared with deionized water, the concentration is 0.5g / ml, and the hydrogenation The amount of sodium aqueous solution is limited with the mol ratio=1: 1.5 of the glucose unit of natural pullulan polysaccharide and sodium hydroxide in the mixed solution;

[0048] (2) sodium chloroacetate aqueous solution and isopropanol are joined in the mixed solution prepared by step (1) simultaneously, under normal pressure, 80 ℃ of reaction 5 hours under stirring; The concentration of described sodium chloro...

Embodiment 3

[0056] The processing steps of the present embodiment are as follows:

[0057] (1) Add isopropanol to the natural pullulan aqueous solution, then add sodium hydroxide aqueous solution, stir at normal pressure and 60°C until a transparent solution is formed; the natural pullulan aqueous solution is prepared with deionized water , the concentration is 0.3g / ml, the volume ratio of the isopropanol to the natural pullulan aqueous solution is 1:9; the sodium hydroxide aqueous solution is prepared with deionized water, and the concentration is 0.2g / ml, and the sodium hydroxide aqueous solution The amount is limited with the mol ratio=1:1.4 of the glucose unit of natural pullulan polysaccharide and sodium hydroxide in the mixed solution;

[0058] (2) sodium chloroacetate aqueous solution and isopropanol are joined in the mixed solution prepared by step (1) simultaneously, under normal pressure, 60 ℃ of reaction 3 hours under stirring; The concentration of described sodium chloroacetat...

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Abstract

The invention relates to a natural pullulan nano drug carrier, which is made of carboxymethylation pullulan and hydrazine under condensation reaction, wherein the condensation reaction degree of carboxyl of the carboxymethylation pullulan with amino of the hydrazine is 60-90%. The carriers with drug are automatically combined in water to form nano particles with particle size ranging from 60nm to 200nm. The preparation method includes the steps: firstly, preparing carboxymethylation pullulan; and secondly, using deionized water to make the carboxymethylation pullulan into solution with concentration being 0.01-0.10g/ml, using deionized water to make hydrazine into solution with concentration being 0.1-0.5g/ml, mixing while adding carboxymethylation pullulan aqueous solution into the hydrazine solution at room temperature and normal pressure, well mixing, adding 1-(3-dimethylamino propyl)-3-ethyl carbodiimide hydrochloride aqueous solution with concentration being 0.5-1g/ml, mixing for reaction for two to six hours, using deionized water for dialyzing to remove micromolecular impurities after reaction is completed, and freeze drying to obtain the natural pullulan nano drug carrier.

Description

technical field [0001] The invention belongs to the field of drug carriers, and in particular relates to a natural pullulan nanometer drug carrier and a preparation method thereof. Background technique [0002] Drug carrier is a kind of drug delivery system. Intelligent nano-drug carrier is beneficial to achieve targeted drug delivery and conquer difficult diseases such as cancer. Due to the characteristics of the tumor tissue, the nanoparticle drug delivery system can be passively concentrated in the tumor site through the EPR phenomenon and absorbed into the cell in the form of endocytosis. In the primary lysosome and secondary lysosome in the cell, because the internal pH value is lower than the pH value of the human blood circulation system, this change in pH makes the drug contained in the nanoparticle drug delivery system separate from it under acidic conditions. , so as to realize the environment-sensitive passive targeted therapy of tumors. Therefore, research on n...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K47/36
Inventor 樊渝江李桦楠边少荃张兴栋
Owner SICHUAN UNIV
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