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Method for detecting insecticide resistance of schistosoma japonicum eggs to praziquantel in vitro

An in vitro detection, praziquantel technology, applied in biological testing, material inspection products, etc., can solve the problems of drug resistance, long time, missed inspection and missed treatment, etc., to achieve the effect of solving on-site detection and monitoring, and solving technical problems

Inactive Publication Date: 2012-07-25
JIANGSU INST OF PARASITIC DISEASES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Long-term and large-scale repeated application of the same insecticide will often lead to drug resistance, so it is necessary to establish methods for on-site monitoring and detection of praziquantel resistance
The current main method of detecting praziquantel resistance is to treat patients on the spot in the epidemic area and evaluate the results of chemotherapy. This method takes a long time, the sequence of patients is low, and there is a phenomenon of missed detection and treatment.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Embodiment 1: Sensitivity determination of eggs of Schistosoma japonicum praziquantel sensitive strains to praziquantel

[0012] Take two 50 mL flasks and transfer them into 5×10 -6 mol / L praziquantel normal saline and a single reference normal saline, add 1000~2000 eggs collected from feces of hosts infected with Schistosoma japonicum Sichuan sensitive strain, put them in a dark environment at 28°C for 24 h, and then place The eggs were transferred into fresh dechlorinated tap water, and the miracidia were hatched under the irradiation of incandescent lamp at 28 °C. Every 30 minutes after the hatching started, the tap water containing miracidia was collected with a 50 mL centrifuge tube, a few drops of Lugol's iodine solution was added and centrifuged, the collected miracidia were observed and counted under a microscope, and the egg hatching rate was calculated. The experiment was repeated three times. The results show that in warp 5×10 -6 After mol / L praziquantel...

Embodiment 2

[0013] Embodiment 2: Sensitivity determination of eggs of Schistosoma japonicum praziquantel sensitive strains to praziquantel

[0014] Take two 50 mL flasks and transfer them into 5×10 -6 mol / L praziquantel normal saline and a single reference normal saline, 1000-2000 eggs collected from the feces of hosts infected with Schistosoma japonicum Anhui sensitive strains were added to the flask, placed in a dark environment at 28°C for 24 h, and then placed The eggs were moved into fresh dechlorinated tap water, and the miracidia were hatched under the irradiation of incandescent lamp at 28°C. After the hatching started, the tap water containing miracidia was collected once every 30 min with a 50 mL centrifuge tube, after adding a few drops of Lugol's iodine solution, centrifuged, the collected miracidia were observed and counted under a microscope, and the egg hatching rate was calculated. The experiment was repeated three times. The results show that in warp 5×10 -6 After mo...

Embodiment 3

[0015] Embodiment 3: Sensitivity determination of eggs of Schistosoma japonicum praziquantel sensitive strain to praziquantel

[0016] Take two 50 mL flasks and transfer them into 5×10 -6 mol / L praziquantel normal saline and a single reference normal saline, 1000-2000 eggs collected from the feces of hosts infected with Schistosoma japonicum Hunan sensitive strains were added to the flask, placed in a dark environment at 28 ℃ for 24 h, and then placed The eggs were moved into fresh dechlorinated tap water, and the miracidia were hatched under the irradiation of incandescent lamp at 28°C. After the hatching started, the tap water containing miracidia was collected once every 30 min with a 50 mL centrifuge tube, after adding a few drops of Lugol's iodine solution, centrifuged, the collected miracidia were observed and counted under a microscope, and the egg hatching rate was calculated. The experiment was repeated three times. The results show that in warp 5×10 -6 After bei...

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Abstract

The invention relates to a method for detecting insecticide resistance of schistosoma japonicum eggs to praziquantel in vitro, belonging to the technical field of antiparasitic drugs. The method comprises the following steps of: collecting 1,000-2,000 eggs from the feces of schistosoma japonicum infected hosts, respectively immigrating the 1,000-2,000 eggs in a flask of 50mL of normal saline containing praziquantel of 5*10-6mol / L and single control normal saline, and placing the eggs in the dark environment for 24h; then immigrating the eggs in fresh dechlorinated tap water, and hatching miracidia under irradiation of an incandescent bulb of 28 DEG C; after hatching starts, collecting tap water containing the miracidia for one time with a centrifuge tube of 50mL every 30 min; centrifuging after adding few drops of Lugol's iodine solution; collecting the miracidia, observing the miracidia under a microscope, counting, and calculating the hatching rate of eggs; setting normal saline control, and repeating for 3 times; detecting the insecticide resistance of the schistosoma japonicum eggs to praziquantel by observing the hatching rate of eggs. The method is fast, simple, convenient, sensitive and reliable and is suitable for fast detecting and monitoring the insecticide resistance of the schistosoma japonicum to praziquantel on site.

Description

technical field [0001] The invention provides an in vitro method for detecting the drug resistance of Schistosoma japonicum eggs to praziquantel, which belongs to the technical field of antiparasitic drugs. Background technique [0002] Schistosomiasis japonicum is a major infectious disease that endangers human health and affects social and economic development. Praziquantel is the only commercially available drug currently used to treat schistosomiasis japonicum. Long-term and large-scale repeated application of the same insecticide will often lead to drug resistance, so it is necessary to establish methods for on-site monitoring and detection of praziquantel resistance. The current main method of detecting praziquantel resistance is to treat patients on-site in the epidemic area and evaluate the results of chemotherapy. This method takes a long time, the sequence of patients is low, and there is a phenomenon of missed detection and treatment. We have established an in v...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50
Inventor 汪伟李洪军梁幼生曲国立邢云天戴建荣
Owner JIANGSU INST OF PARASITIC DISEASES
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