Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for enhancing ectogenesis of sheep oocyte

An oocyte, in vitro development technology, applied in artificial cell constructs, germ cells, animal cells, etc., can solve problems such as the developmental ability of few sheep oocytes

Active Publication Date: 2013-06-05
INNER MONGOLIA SAINUO GRASSLAND SHEEP IND
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned existing methods are used in cattle, mice, and pigs, but there are few reports on improving the developmental ability of sheep oocytes

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0022] Pick up sheep ovaries that were slaughtered within 30 minutes, put them in physiological saline containing 1000IU / ml penicillin and 1000IU / ml streptomycin at a temperature of 30°C, wash 4 times with normal saline within 3 hours; 100 μMol / L FSK and 500 μMol / L IBMX egg extraction fluid, a 10ml syringe with a 9-gauge needle to aspirate follicles with a size of 6 mm on the surface of the ovary, and the recovered follicle fluid in the syringe was placed in a 60 mm dish and examined under a microscope Pick out the oocytes, place them in the egg extraction solution for 2 hours, wash 3 times with the in vitro maturation solution containing 20 μMol / L CIL, and place them in the CO 2 Incubate in the box for 2 hours, CO 2 The meteorological condition of the box is 5% CO 2 , 95% air, the temperature is 38.6 ℃. Then wash 3 times with CIL-free in vitro maturation solution, CO 2 Cultivate in the box for 24 hours, wash 4 times with in vitro fertilization fluid, add 30 pieces / drop int...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for enhancing the ectogenesis of sheep oocyte. The method adopts an adenylate cyclase activator Forskolin (FSK), phosphodiesterase inhibitor cilostamide (CIL) and 3-isobutyl-1-methylxanthine (IBMX), treats the sheep oocyte in a combining manner and improves the capability of the ectogenesis of the sheep oocyte. The method comprises the following detailed steps of: picking the ovary of the killed sheep, putting the ovary of the killed sheep into normal saline for cleaning for 3-4 times; pumping the 2-8mm follicles on the surface of the ovary by using a 10ml syringe which absorbs 1ml ovum-pumping liquid containing 100mu mol / L FSK and 500mumol / L IBMX and is provided with a number-9 needle head, and injecting the follicular fluid recovered in the syringe into a35-60mm vessel; picking the oocyte under a microscope, putting the oocyte into the pumped follicular fluid, cleaning the oocyte for three times with in-vitro mature fluid containing 20mu mol / L CIL, and putting the oocyte in a CO2 incubator for culture; then cleaning the oocyte for three times with in-vitro mature fluid which does not contain CIL, putting the oocyte in the CO2 incubator for culture and adding into in-vitro fertilization fluid drop; unfreezing the seminal fluid with water bathing, and commonly incubating with the oocyte; and calculating the cleavage rate after 24 hours and calculating the blastocyst rate after 168 hours.

Description

Technical field [0001] The invention involves the in vitro development of sheep and oocytes. The adenolate cycle enzyme activation agent Forskolin (FSK), phosphate diacease inhibitor Cilostamide (CIL) and 3-bentite-1-methyl tracerine 3-ISOBUTYL-Methylxanthine (IBMX), combined with the treatment of sheep and egg mother cells, can significantly improve its in vitro development ability. Background technique [0002] In the animal husbandry industry, the use of external fertilized technology can produce test tube embryos in the form of factory, thereby providing more high -quality embryos for domestic animal embryo transplantation, and promoting the nickname of livestock.In vitro fertilization technology, it mainly includes three links: ovarian mother cell maturity, sperm acquisition of fertilization, and in vitro cultivation of embryo body. [0003] For mammalian oocytes, high -concentrations of adenosine (CAMP) of cyclopensions (CAMP) can inhibit the division of degradation, thereb...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/075C12N5/071
Inventor 黄俊成汪立芹林嘉鹏赵云程唐森玛依拉
Owner INNER MONGOLIA SAINUO GRASSLAND SHEEP IND
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com