Multi-gene multi-zone specific capture method

Abstract

The invention discloses a kit for capturing a specific nucleic acid target with multiple genes in multiple zones. The kit is characterized in that the kit comprises a first primer, a capture component and a second primer, wherein the first primer is used for performing first unidirectional PCR (polymerase chain reaction) amplification on a target gene region sequence to be captured, and one end of the first primer is connected with a first linker DNA (deoxyribonucleic acid) fragmentation marked with biotin and is in complementary combination with the target gene region sequence to be captured to form an oligonucleotide single stranded sequence; the capture component comprises an avidin and a solid-phase carrier combined with the avidin; the avidin is used for capturing a target gene region sequence after the first PCR amplification and combining with the avidin on the target gene region sequence after the first PCR amplification; and the second primer is used for performing second unidirectional PCR amplification on the target gene region sequence after the combination with the avidin, and one end of the second primer is connected with a second linker DNA fragmentation and is in complementary combination with the target gene region, and is opposite to the direction of the PCR amplification performed by the first primer. The kit has good specificity, high homogeneity and high coverage rate and is simple to operate.

Description

technical field [0001] The invention belongs to the technical field of gene sequencing, and in particular relates to a specific capture method for multiple genes and multiple regions. Background technique [0002] In today's world, nucleic acid sequencing technology has made rapid progress, and the Human Genome Project and the genome sequences of many animals, plants and microorganisms have been sequenced successively. Sequencing technology has gradually developed from the original first-generation Sanger sequencing method to high-throughput sequencing technology. High-throughput sequencing technology can simultaneously sequence millions or even tens of millions of identical or different short DNA fragments (60-500bp), and generate huge sequence data. However, the processing of these huge data poses serious challenges to scientists. For a genome sequence, it may only take two or three days to complete the sequence, but it takes a week or more to process the data. This gre...

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Problems solved by technology

[0014] The purpose of the present invention is to provide a kit for multi-gene and multi-region capture of specific nucleic acid targets, which solves various deficiencies in the gene capture technology in the prior art

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