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Fluorescence quantitative PCR (polymerase chain reaction) qualitative detection method for rabbit-derived fiber composition

A qualitative detection and fluorescence quantitative technology, applied in the field of bioengineering, can solve the problems of not very clear scales, time-consuming and laborious detection, etc., and achieve the effect of false sensitivity and good specificity.

Inactive Publication Date: 2014-03-12
上海出入境检验检疫局工业品与原材料检测技术中心 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, under a general optical microscope, the scales are not very clear, and it takes time and effort to detect them.

Method used

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  • Fluorescence quantitative PCR (polymerase chain reaction) qualitative detection method for rabbit-derived fiber composition
  • Fluorescence quantitative PCR (polymerase chain reaction) qualitative detection method for rabbit-derived fiber composition
  • Fluorescence quantitative PCR (polymerase chain reaction) qualitative detection method for rabbit-derived fiber composition

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Embodiment 1

[0055] 1. Design of probes and primers

[0056] Common fur fox species on the market include arctic fox and red fox. Taking into account the same part of the 12srRNA sequence of the mitochondrial gene of the two, compare it with the rabbit sequence, and select the appropriate sequence for primer probe design.

[0057] The product size for the rabbit-derived component was 68 bp. The primer and probe sequences are as follows:

[0058] RabF: 5'-CGAACCCTAAAAAGGAGCAAAA-3' (SEQ ID NO: 1)

[0059] RabR: 5'-GGGCTACACCTTGACCTAACGT-3' (SEQ ID NO: 2)

[0060] Prab: FAM-AAGCTCAATTACCACCGTAA-MGB (SEQ ID NO: 3)

[0061] The product size for the fox-derived component was 70 bp. The primer and probe sequences are as follows:

[0062] FoxF: 5'-ACAAAACAATTCGCCAGAGAACT-3' (SEQ ID NO: 4)

[0063] FoxR: 5'-GGATATAAAGCACCGCCAAGTC-3' (SEQ ID NO: 5)

[0064] Pfox: FAM-TAGCAACAGCTTAAAACT-MGB (SEQ ID NO: 6)

[0065] The product size for the sheep-derived component was 61 bp. The primer and pro...

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Abstract

The invention discloses a fluorescence quantitative PCR (polymerase chain reaction) qualitative detection method for a rabbit-derived fiber composition. The fluorescence quantitative PCR qualitative detection method comprises the following steps of: step 1, carrying out PCR amplification by taking DNA (deoxyribonucleic acid) of rabbit hair as a template; step 2, detecting a fluorescence signal of an amplification product, wherein a reaction system for PCR amplification contains amplified rabbit-derived fiber composition specificity primer pairs and a rabbit-derived fiber composition specificity probe. The fluorescence quantitative PCR qualitative detection method disclosed by the invention can simply, conveniently and quickly detect and identify whether the rabbit-derived fiber composition exists in textile or not, has good specificities on the designed specificity primers and specificity probes and excellent sensitivity, and can detect fox hairs with the proportion of 1%(wt).

Description

technical field [0001] The invention belongs to the field of bioengineering, and specifically relates to a qualitative detection method of rabbit-derived fiber components by fluorescent quantitative PCR. Background technique [0002] Rabbit fur and fox fur are the favorites in the autumn and winter market. Fur has long flourished in the international market because of its warmth, comfort and beauty. In addition, there are also rabbit down and fox down fabric products on the market. In general, fox fur is longer, denser and softer than rabbit fur, and the cost of feeding is also higher. The price of fox fur is much higher than that of rabbit fur products. In recent years, with the improvement of breeds, rabbit fur has gradually become slender and soft, such as Angora rabbit, rex rabbit, etc., which makes the identification of fur more difficult. Rabbit fur and fox fur all have a medulla layer, and a very small number of fine hairs have no medulla, and small lattice-like ca...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/686C12Q2561/101
Inventor 杨娟费静唐敏峰陆维明王小平叶尚华高友军刘锦瑞
Owner 上海出入境检验检疫局工业品与原材料检测技术中心