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Antiviral agent containing coriolus versicolor polysaccharide and preparation method thereof

An antiviral agent, Yunzhi polysaccharide technology, applied in the directions of disinfectants, botanical equipment and methods, biocides, etc., can solve problems such as affecting crop yield and quality, no effective control agents, growth and development stagnation, etc., and achieve good development. Potential application, good preventive and therapeutic effects, effects on crop growth promotion

Inactive Publication Date: 2012-09-12
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After the crops are infected with CMV, the incidence and epidemic speed are extremely fast, and the growth and development stagnate, which seriously affects the yield and quality of crops and causes serious economic losses.
[0003] At present, the control of viral diseases is mainly through planting non-toxic seeds, virus-free seedlings and propagation materials, removing susceptible plants and their virus-transmitting media, and strengthening cultivation management. However, there is no effective control agent in production so far.

Method used

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  • Antiviral agent containing coriolus versicolor polysaccharide and preparation method thereof
  • Antiviral agent containing coriolus versicolor polysaccharide and preparation method thereof
  • Antiviral agent containing coriolus versicolor polysaccharide and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation Embodiment 1

[0028] Preparation Example 1: Preparation of 5% Yunzhi Polysaccharide Aqueous

[0029] Weigh 5g of Yunzhi polysaccharide and dissolve it in appropriate amount of water, then add 2g~3g of surfactant, 1g~3g of antifreeze, 0.1g~0.5g of preservative, 1~3% of stabilizer, and make up to 100g of water to make 5 % Yunzhi polysaccharide water agent.

[0030] In this embodiment, octylphenol polyoxyethylene ether is selected as the surfactant, ethylene glycol is selected as the antifreeze, liquid barium zinc is selected as the stabilizer, and sodium benzoate is selected as the preservative.

preparation Embodiment 2

[0031] Preparation Example 2: Preparation of 7% Yunzhi Polysaccharide Aqueous

[0032] Weigh 7g of Yunzhi polysaccharide and dissolve it in appropriate amount of water, then add 2g~3g of surfactant, 1g~3g of antifreeze, 0.1g~0.5g of preservative, 1~3% of stabilizer, and make up to 100g of water to make 7 % Yunzhi polysaccharide water agent.

[0033] In this embodiment, nonylphenol polyoxyethylene ether is selected as the surfactant, propylene glycol is selected as the antifreeze, liquid calcium zinc is selected as the stabilizer, and sorbic acid is selected as the preservative.

[0034] Of course, the preservative in the above embodiments can also be selected from one of benzoic acid, potassium sorbate, and calcium propionate, and a qualified Yunzhi polysaccharide liquid can be obtained.

experiment Embodiment 1

[0035] Experimental Example 1: Research on antiviral activity of Yunzhi polysaccharide water

[0036] 2.1 Inactivation test of Yunzhi polysaccharide water on CMV in vitro

[0037] The dead spot method was used to determine the inactivation effect of different concentrations of Yunzhi polysaccharide water on CMV in vitro, and the results are shown in Table 1. It can be seen from Table 1 that the Yunzhi polysaccharide aqueous solution with a concentration of 500mg / L showed a significant passivation effect on CMV. After passivation treatment for 0.5h, 1h, and 2h, the inhibition rate of CMV was 57.32%, respectively. 73.71%, 76.89%, the inhibitory effect is better than that of the control agent; and Yunzhi polysaccharide water agent has a time effect on the passivation of CMV, and the passivation effect gradually increases with the prolongation of the passivation time.

[0038] Table 1: The results of the inactivation test of CMV in vitro by Yunzhi polysaccharide water and control

[0039...

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Abstract

The invention discloses an antiviral agent containing coriolus versicolor polysaccharide, comprising 5-7 wt% of coriolus versicolor polysaccharide, 2-3wt% of surfactant, 1-3 wt% of antifreeze agent, 0.1-0.5 wt% of preservative, 1-3 wt% of stabilizing agent, and the balance of water, wherein the summation of the weight percentages of the raw materials is 100 wt%. The antiviral agent containing coriolus versicolor polysaccharide is accord with the quality requirements of pesticide preparations, has good control effect on Cucumber mosaic virus, CMV, has growth promoting effect on crops, can improve the disease resistance of the crops. In addition, the antiviral agent disclosed herein has high safety for human, animal and environment, thus the antiviral agent is an ideal biopesticide for developing efficient, green and ecological agriculture.

Description

Technical field [0001] The invention relates to a plant virus control agent and its preparation, in particular to an antiviral agent containing Yunzhi polysaccharide and a preparation method thereof. The prepared antiviral agent can effectively prevent Cucumber mosaic virus disease (Cucumber mosaic virus). , CMV). Background technique [0002] Plant virus diseases are a variety of diseases in agricultural production that are common and severely harmful. Among them, Cucumber mosaic virus (Cucumber mosaic virus, CMV) can infect more than 1,000 species of monocotyledonous and dicotyledonous plants, and can be transmitted by aphids and seeds. It is the most host range, the most widely distributed, and the most economically important. One of the sexual plant viruses. After the crops are infected with CMV, the epidemic rate is extremely fast, and the growth and development are stagnant, which seriously affects the yield and quality of crops and causes serious economic losses. [0003]...

Claims

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Application Information

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IPC IPC(8): A01N65/00A01P1/00
Inventor 安德荣牛小义
Owner NORTHWEST A & F UNIV
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