Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for producing pectate lyase by two-section pH (potential of hydrogen) control

A pectinase and alkaline technology, which is applied in the field of biological enzyme preparation, can solve the problems of unsuitable for industrial production and application, low activity of alkaline pectinase, and high cost, so as to save the chemical degumming process, shorten the fermentation cycle, The effect of improving quality

Active Publication Date: 2013-05-29
SHANDONG UNIV
View PDF9 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the deficiencies in the prior art that the activity of alkaline pectinase is low, the cost is high, and it is not suitable for industrialized production and application, the purpose of the present invention is to provide a method for producing alkaline pectinase by using two-stage pH control fermentation

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for producing pectate lyase by two-section pH (potential of hydrogen) control

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Bacillus subtilis CCTCC NO:M200038 (applicant deposited in China Center for Typical Cultures (CCTCC) on November 20, 2000, see ZL01106844.2 for related patents) was activated on an inclined plane, and the bacterial lawn was inoculated to the medium containing the seeds Cultivate in shake flasks, the culture conditions are: 300ml Erlenmeyer flask liquid volume is 50ml, fermentation temperature 37 DEG C, 200rpm shaking culture 8h. Inoculate the initial fermentation medium with an inoculum size of 10%, the pH is controlled at 5.6-6.0, the temperature is 33-34°C, and the ventilation volume is 2.5-4.5Lmin -1 . The starch in the feed medium was liquefied with mesophilic amylase, and the amount of enzyme added was 32.5U g -1 , the liquefaction temperature is 55°C, and the liquefaction time is 30min. After initial fermentation to 16 hours, add pretreated feeding medium according to 20% of the volume of the initial fermentation medium, control the pH at 5.1-5.5, control the te...

Embodiment 2

[0035] The fermentation method and conditions were the same as in Example 1 except for the following changes. The pH before feeding was controlled to be 6.0-6.4, and the pH was controlled to be 5.5-5.9 after feeding. Through experimental determination, the crude enzyme liquid obtained by fermentation has the highest alkaline pectinase activity of 743Uml -1 . Fermentation process such as figure 1 shown.

Embodiment 3

[0037] The fermentation method and conditions were the same as in Example 1 except for the following changes. Before feeding, the pH was controlled to be 6.4-6.8, and after feeding, the pH was controlled to be 5.9-6.3. According to the experiment, the highest alkaline pectinase activity of the fermented crude enzyme liquid is 660U ml -1 . Fermentation process such as figure 1 shown.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for producing pectate lyase by means of fermentation under two-section pH (potential of hydrogen) control. As requirements on physicochemical environments and nutritional statuses (pH can be directly or indirectly reflected) are different when cells are in merisis in the early stage of fermentation and produce enzyme in the middle and late stage of fermentation, pH of fermenting liquid is controlled in a two section manner in an online feedback pH controlling method before and after feeding, and pectate lyase activity and production efficiency thereof are improved. Experiments show that fermentation coarse enzyme fluid of the pectate lyase with the poly galacturonic acid cracking enzyme activity of 743Uml-1 is obtained by the method, and the highest volumeproduction efficiency reaches 24U(mL*h)-1. The pectate lyase produced by the method is low in cost, short in fermenting period, high in equipment utilization rate, simple in process, and applicable to large-scale industrial production.

Description

technical field [0001] The invention relates to a method for producing alkaline pectinase, in particular to a method for producing alkaline pectinase by segmentally controlling the pH before and after feeding, and belongs to the technical field of biological enzyme preparation. Background technique [0002] Pectinase (pectinases) is a general term for a large class of enzymes that can decompose pectin, a component of plant cell walls. From the perspective of decomposition methods, pectinases can be divided into pectinases and pectin lyases. The former mainly includes pectinases. Gluease (PPase), pectin esterase (PE), oligogalacturonan hydrolase, polymethylgalacturonase (PMG), polygalacturonase (PG); the latter mainly includes poly Galacturonate lyase (PGL) and polymethylgalacturonate lyase (PMGL). Alkaline pectinase, which is often called by people, generally refers to polygalacturonic acid lyase in pectin lyase. Pectin lyase cleaves the pectin polymer by trans-elimination...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/88C12R1/125
Inventor 李雪芝邹谋勇赵建曲音波
Owner SHANDONG UNIV