Cuff-like vessel imbedding method used for portal vein reconstruction of rat liver transplantation

Abstract

The invention provides a cuff-like vessel imbedding method used for portal vein reconstruction of rat liver transplantation, named portal vein branch suspension way. The method comprises the steps of: respectively embedding silk yarns to serve as traction lines through initial segments of left and right branches of free portal vein during excision of a receptor liver; after a superior vena cava and an inferior vena cava of the receptor liver are reconstructed, suspending the left and right branch traction lines of the portal vein to the head and the external side of the rat and keeping a certain tensile force to enable the left and right branches of the portal vein to be fixed at suitable positions and angles; shearing off the front wall of the portal vein at a junction part of the left and right branches of the receptor portal vein and trimming the front wall into an enlarged U-shaped opening; embedding a cuff-like vessel of a donor portal vein into a receptor portal vein cavity through the U-shaped opening at the junction part of the branches of the receptor portal vein and bundling the cuff-like vessel annularly by the silk yarns. The cuff-like vessel imbedding method used for the portal vein reconstruction of the rat liver transplantation, disclosed by the invention, has the advantages of being easy, rapid and reliable to operate and being capable of shortening training time and saving cost when the cuff-like vessel imbedding method is used for portal vein reconstruction of the rat liver transplantation.

Description

technical field [0001] The invention relates to a portal vein reconstruction method for rat liver transplantation, in particular to a cuff tube insertion method for portal vein reconstruction of rat liver transplantation, which is suitable for application in the field of organ transplantation animal model construction. Background technique [0002] Since Kamada et al. introduced the "sleeve method" vascular reconstruction technique into rat liver transplantation in 1979, the "double-sleeve method" has gradually become the standard surgical procedure for rat liver transplantation models, and is widely used in transplantation immunity, small grafts, and deficient organs. Blood reperfusion injury and other research fields have become an important tool for basic research on organ transplantation. [0003] "Double-sleeve" liver transplantation in rats is a multi-step, complex and challenging surgical process. It is not easy to establish a stable "double-sleeve" rat liver transpla...

AI Technical Summary

Problems solved by technology

There are many problems in these two methods. The micro-closer method has high technical requirements. For example, the fixation and improper position of the micro-closer may easily make it difficult to approach the two sides of the blood vessel to be anastomosed to complete the reconstruction, and the portal vein trunk is turned over. When the vein wall is damaged, it is easy to cause dry tearing of the portal vein or it is easy to fall off after being placed in the portal vein cavity. Short-term training is not easy to succeed; the suture suspension method of the posterior wall of the portal vein first sutures the feet on both sides of the posterior wall of the portal vein, and then suspends the traction. It is also easy to cause the slender main vein wall to tear during the insertion of the portal cuff tube or the lateral foot of the sutured posterior wall of the portal vein to tear off, requiring suture suspension again, resulting in repeated cuff tube insertion and prolonging the anhepatic period. Even cause failure of portal vein reconstruction or surgery

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