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Separation and purification method of endophytic fungi from ginkgo biloba

A technology of endophytic fungi and ginkgo, applied in the field of microorganisms, can solve the problems of high toxicity, long residual effect period, blind and excessive application of pesticides, etc.

Active Publication Date: 2014-01-15
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the interdependence and mutual restraint among natural organisms, excessive reliance on the poisonous effect of chemical pesticides has led to the phenomenon of blind and indiscriminate use of pesticides in some areas, especially the use of some pesticides with long residual effects and high toxicity, resulting in a series of food safety issues. , human health and living environment issues

Method used

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  • Separation and purification method of endophytic fungi from ginkgo biloba
  • Separation and purification method of endophytic fungi from ginkgo biloba
  • Separation and purification method of endophytic fungi from ginkgo biloba

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Embodiment 1

[0071] The specific operation steps of the separation and purification method of ginkgo endophytic fungus are as follows:

[0072] (1). Ginkgo tissue preparation: collect the roots, stems and leaves of Ginkgo;

[0073] (2). Surface disinfection and sterility testing of ginkgo tissue: Soak the roots collected in step (1) in 70% alcohol for 1 min and in 0.2% sodium hypochlorite solution for 2 min; Soak in 70% alcohol for 50s, soak in 0.2% sodium hypochlorite solution for 90s; the collected leaves are soaked in 70% alcohol for 40s, and soak in 0.2% sodium hypochlorite solution for 60s respectively; the soaked roots, Rinse the stems and leaves with sterile water for 3 times, and dry them in the shade to obtain sterilized roots, stems, and leaves; cut the sterilized roots, stems, and leaves into 0.5cm×0.5cm-sized root pieces and stems with a sterile scalpel block, leaf block; then a part of the root block, stem block, and leaf block are directly planted on the first potato dextros...

Embodiment 2

[0091] The specific operation steps of the separation and purification method of ginkgo endophytic fungus are as follows:

[0092] (1). Ginkgo tissue preparation: collect the roots, stems and leaves of Ginkgo;

[0093] (2). Surface disinfection and sterility testing of ginkgo tissue: Soak the roots collected in step (1) in 70% alcohol for 1 min and in 0.2% sodium hypochlorite solution for 2 min; Soak in 70% alcohol for 50s, soak in 0.2% sodium hypochlorite solution for 90s; the collected leaves are soaked in 70% alcohol for 40s, and soak in 0.2% sodium hypochlorite solution for 60s respectively; the soaked roots, Rinse the stems and leaves with sterile water for 3 times, and dry them in the shade to obtain sterilized roots, stems, and leaves; cut the sterilized roots, stems, and leaves into 0.5cm×0.5cm-sized root pieces and stems with a sterile scalpel block, leaf block; then a part of the root block, stem block, and leaf block are directly planted on the first potato dextros...

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Abstract

The invention relates to a separation and purification method of endophytic fungi from ginkgo biloba. The separation and purification method comprises the following operation steps of: (1) preparing ginkgo biloba tissues: collecting roots, stems and leaves of the ginkgo biloba and preserving under the condition that the temperature is 4 DEG C; (2) performing surface disinfection and sterile detection on the ginkgo biloba tissues; and (3) performing separation culture on the endophytic fungi from the ginkgo biloba tissues. The endophytic fungi from the ginkgo biloba, described by the invention, is named as Fusariumsolani T-7 and currently collected in China General Microbiological Culture Collection Center designated by State Intellectual Property Office, the collection data is July 20, 2011, and the collection number is CGMCC No. 5089. The endophytic fungi from the ginkgo biloba, disclosed by the invention has the microbiology position of the Fusariumsolani T-7 with broad-spectrum antimicrobial activity, and the strain is simultaneously found to have an obvious effect of inhibiting the growth of mycelia against pathogenic fungi of six tested plants. According to the separation and purification method disclosed by the invention, resources of the plant endophytic fungi are utilized to get a novel agricultural antibiotic type natural component for resisting diseases caused by the plant pathogenic fungi.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, in particular to a microorganism whose fermented product has the activity of inhibiting plant pathogenic fungi. Background technique [0002] The main means of controlling crop diseases, insect pests and weeds in the process of agricultural production is the control of chemical pesticides, which play a positive role in reducing the damage of diseases, insect pests and weeds and ensuring high grain yields. However, due to the interdependence and mutual restraint among natural organisms, excessive reliance on the poisonous effect of chemical pesticides has led to the phenomenon of blind and indiscriminate use of pesticides in some areas, especially the use of some pesticides with long residual effects and high toxicity, resulting in a series of food safety issues. , human health and living environment issues. At present, the hazards of chemical control are gradually being recognized by peop...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12R1/77
Inventor 花日茂郭正彦柏钰操海群吴祥为李学德唐俊
Owner ANHUI AGRICULTURAL UNIVERSITY