Method for extracting DNA (deoxyribonucleic acid) by alkaline cracking

Abstract

The invention provides a method for extracting DNA (deoxyribonucleic acid) by alkaline cracking. The method comprises the following steps of: 1, putting testing material into NaOH (sodium hydroxide) solution of which the concentration is 0.05-0.6M; 2, carrying out incubation on solution, wherein the incubation temperature is 90-99 DEG C and the incubation time is 5-10 minutes; 3, oscillating the incubated solution in an oscillator to oscillate; 4, adding Tris-HCl (tris(hydroxymethyl)aminomethane hydrochloride) solution of which the concentration is 0.01-0.1M and the pH value is 5-8 and carrying out centrifugal oscillation, and 5, freezing and storing extract at the temperature of 20 DEG C. Compared with the prior art, the requirements of existing various conventional forensic biological test materials can be met by changing parameters such as the concentration, the pH value, the incubation time, the incubation temperature and the like of reagent, and automatic DNA extraction work can be carried out at the same time by the same equipment under the same experiment conditions.

Description

technical field [0001] The invention relates to a method for extracting DNA, in particular to a method for extracting DNA by alkaline lysis. Background technique With the continuous deepening of DNA database construction in my country, the amount of DNA data in the national library has increased rapidly, and the national consumption of biological testing materials has also increased day by day. According to statistics, the country needs to invest about 200 million in DNA testing funds a year. How to establish a new DNA testing method on the premise of ensuring the accuracy of DNA testing results, and greatly reduce the cost of DNA database biological testing materials is of great significance. At present, the inspection methods of biological samples in DNA database are mainly divided into two categories: The first type is to use Chelex-100 or magnetic beads to extract DNA from biological samples and then amplify it. The second type is to directly amplify with a direct...

AI Technical Summary

Problems solved by technology

However, the existing alkaline lysis method also has some shortcomings, for example: the DNA of biological samples cannot be stored at room temperature; it is not suitable for all biological samples; the extraction methods of different types of biological samples are different

These deficiencies make it unable to meet the needs of all kinds of conventional forensic biological samples for automatic DNA extraction at the same time under the same equipment and experimental conditions.

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